| Objective:By culturing KATO? human gastric cancer cell lines in vitro and treating KATO? gastric cancer cell lines with different hyperthermia temperatures,simulating tumor hyperthermia(41?-43?)environment,observed at 37?,40?,41?,42?,43? environment and the presence or absence of Tranilast,the proliferation of cancer cells and the change of TGF-?mRNA expression level in cells,so as to find more for hyperthermia and Tranilast in the treatment of gastric sclerosis Targeted experimental basis and clinical treatment provide new ideas.Methods:Taking 10 sterile plastic 6-well plates and dividing them into experimental group and control group,with 5 well plates in each group,and mark 37?,40?,41?,42? and 43? on the cover of each group.Then,KATO? human gastric cancer cell suspension was inoculated into the prescribed wells of 10 6-well plates at a density of 2.0 mL/well and a density of 5 × 10 4 cells/mL.Nistel)ratio 1 mL of Tranilast(tranilast)solution at a concentration of 1.0 mmol/L was added to the wells of the experimental group,and 1 mL of IMDM complete culture medium was added to the control group,which were placed at 37?,40?,41?,42? and 43?.After 6 hours of incubation in a CO2 incubator with a volume fraction of 0.05%.Finally,Luna cell counter and RT-PCR were used to detect the proliferation of KATO? human gastric cancer cells and the expression of TGF-?mRNA when cultured at different temperatures under the intervention of tranilast.Value,the mean± standard deviation(X±S)is used to represent the experimental data.After the data is tested for normality and homogeneity of variance,the single factor analysis of variance method is used to measure Vertical and horizontal comparison analysis was performed at 37?,40?,41?,42?,and 43??Results:(1)Experimental group:The number of cells in each well plate was measured by Luna cell counter under the combined action of 37?,40?,41?,42?,and 43? and the use of Tranilast,which was approximately 7.13±0.05×105 cell/mL?5.33±0.12×105 cell/mL?3.70±0.02×105 cell/mL?2.28±0.04×105 cell/mL?1.13±0.06×105 cell/mL;The relative expression of TGF-? mRNA was measured by RT-PCR.They are 0.66±0.02?0.51±0.02?0.31±0.01?0.22±0.01?0.10±0.02.(2)Control group:Under the action of 37?,40?,41?,42? and 43?,the number of cells in each well plate measured by Luna cell counter is about 9.20±0.17×105 cell/mL?6.19±0.06×105 cell/mL,4.08±0.05×105 cell/mL,3.13±0.12 × 105 cell/mL,2.18±0.04 × 105 cell/mL,and the relative expression levels of TGF-? mRNA measured by RT-PCR were 0.85±0.03?0.63±0.02?0.40±0.02?0.31±0.02?0.23±0.01?(3)By comparing the number of cells and the expression of TGF-?mRNA in the experimental group and the control group at the same temperature by horizontal comparison,it was found that the number of cells and the relative expression of TGF-?mRNA in the experimental group were lower than the control group at each temperature(P<0.05)The difference was statistically significant.(4)By longitudinally comparing the number of cells and the expression of TGF-?mRNA in the control group at different temperatures of 37?,40?,41?,42?,and 43?,it was found that as the temperature increased,the number of cells in the control group and The expression levels gradually decreased(P<0.05),and the difference was statistically significant.(5)By comparing and analyzing the cell numbers and TGF-? mRNA expression in the experimental and control groups at different temperatures of 37?,40?,41?,42?,and 43?,it was found that with the increase of temperature,the experimental group and the control The number of KATO? cells and the relative expression of TGF-? mRNA in the group showed a gradual decrease,and both the number of cells and the expression of TGF-? reached the lowest value at 43?.The difference was statistically significant(P<0.05).The number of cells and the expression of TGF-?mRNA in the experimental group after Nistel at each temperature were lower than those in the control group at the same temperature,and the differences were statistically significant(P<0.05).Conclusion:1.Tranilast has the effect of inhibiting the growth and expression of TGF-? in KATO? gastric scleral cancer cells.2.Temperature control(40?-43?)can inhibit the growth of KATO ?gastric cancer cells and the expression of TGF-?,and 43? is the optimal temperature for hyperthermia.3.When temperature control(40?-43?)combined with Tranilast and hyperthermia acts alone on KATO? gastric cancer cells,the former is more significant in inhibiting the growth of KATO? gastric cancer cells and reducing the expression of TGF-? ? is the optimal temperature for hyperthermia. |
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