New Perylenediimides For Recognition Of Biothiols And Fluoride Ions

In recent years,cancers which have high morbidity and high mortality have always annoyed medical staff and patients.Due to the malignant proliferation and rapid metastasis of tumor cells,it has become the biggest obstacle to treatment.Traditional treatment methods such as surgical resection and radiation Both treatment and chemotherapy bring huge side effects to the patient's body.Therefore,the development of new minimally invasive treatment methods with high cure rate has become an urgent problem to be solved,and at the same time is facing huge challenges.Photodiagnostic reagents are divided into photodiagnostic reagents and phototherapy reagents.The photodiagnostic methods mainly include photoacoustic imaging(PA),fluorescence imaging and X-ray computed tomography(CT)imaging.In order to improve the diagnosis rate of cancer,photodiagnostic reagents play a crucial Important role;as a new type of cancer treatment,phototherapy mainly includes photodynamic therapy and photothermal therapy.Phototherapy agents mainly include graphene nanoparticles,chalcogenide nanoparticles,gold nanoparticles,etc.However,due to the low oxygen environment of tumors and The rapidly generated heat resistance will limit the efficiency of photodynamic therapy and the effect of photothermal therapy,respectively.In the process of combined photodynamic and photothermal treatment,the heat generated by the photodiagnostic reagent will improve the oxygen concentration near the tumor,and the active oxygen component it generates will also kill those heat-resistant tumor cells.Therefore,it is imminent to develop an effective photodiagnostic reagent.This paper designs and synthesizes two kinds of photodiagnostic reagents,which are characterized by ultraviolet absorption spectrum,fluorescence emission spectrum,nuclear magnetic hydrogen spectrum,etc.The specific contents are as follows:1.A fluorescent diagnostic reagent PDI-S-S-Fc with peryleneimide as fluorophore,ferrocene(Fc)as quencher and disulfide bond(S-S)as thiol recognition site was designed and synthesized.Spectral characterization,because of the presence of ferrocene,the fluorescence of the diagnosis and treatment reagents was quenched,which verified the PET process;and the probes had a good response to biomercaptans(GSH,Cys,Hcy,etc.).Increase,the fluorescence gradually increases,the detection limit is 2.29 × 10-7 M.2.Compound L was designed and synthesized.The ability of fluorescent diagnostic reagents to recognize anions was studied by fluorescence emission spectroscopy.Compound L has a specific response to F-.With the continuous addition of F-,fluorescence is quenched.When the multiple is 3.6 eq,saturation is reached and the detection limit is 6.0 × 10-8 M.