Preparation Of PhVEGF Genetic Coated Interventional Device And Its Application In Endovascular Treatment

Background and PurposeIntracranial aneurysm is a common cerebrovascular disease with a incidence rate of 2%to 7%which has a high mortality and disability rate.Endovascular embolization,which is a mainly treatment of intracranial aneurysms has been recommended as the first choice because of its low complication rate and high prognosis rate.In recent years,the appearance of Flow Diverter has brought new method for the treatment of intracranial aneurysms.Unfortunately,there are some defects in Flow Diverter,including excessively invading the parent artery,secondary thrombosis and so on.And antiplatelet drug,which could interfere with the formation of thrombus in aneurysm sac is taken to prevent thrombus before implantation.How to reduce the influence of interventional device on parent artery and reduce the restenosis rate of parent artery has become an important problem to be solved.A new interventional device,which can accelerate the healing of aneurysms,promote the reendothelialization of parent artery and have the function of antithrombotic is an urgent need at present.Vascular Endothelial Growth Factor(VEGF),which can promote endothelial cell division and proliferation,plays an important role in repairing vascular endothelial cell damage and promoting the formation of parent artery.In this study,VEGF genetic coated Flow Diverter is intended to explore the therapeutic effect of the treatment of rabbit cerebral wide-neck aneurysms and the mechanism of VEGF gene on vascular endothelial cells.MethodsThe primer was designed to prepare the target gene and vector according to the sequence of human VEGF,and the successfully constructed recombinant plasmid was used to coat Tubridge.10 rabbits,which had elastase-induced wide-neck aneurysms were divided into an experimental group and a control group,with 5 rabbits in experimental group and 5 in control group.VEGF genetic coated Tubridge and naked Tubridge were implantation,respectively.Clinical symptoms,imaging and pathological characteristics of the two groups after implantation were used to explore the mechanism and effect of the VEGF genetic coated Tubridge in the treatment of aneurysm.ResultsNine rabbits were successfully constructed with wide-neck aneurysms at the carotid artery.The immediately complete occlusion rate of the experimental group after implantation was 33.4%(1/3),and was 0 in the control group.At 8-week follow-up,the complete occlusion rate of the experimental group was 50%(1/2),and was 0 in the control group.ConclusionsThe model of rabbit cerebral wide-neck aneurysms induced by elastase is simple,stable and reliable,and can be used for preclinical experiments of interventional devices for the treatment of aneurysms.VEGF genetic coated Tubridge had a higher occlusion rate and fewer complications in the treatment of rabbit cerebral wide-neck aneurysms than naked Tubridge.It may play a certain role in the repair of vascular endothelium and reduce the damage of interventional device to the vessel.And it has been proved that the VEGF genetic coated Tubridge can promote the reendothelialization of vascular endothelium.