| Objective: To investigate the protective effect and potential mechanism of Coptis chinensis polysaccharide on renal injury in DM rats.Methods: The DM rat model was established by intraperitoneal injection of 60 mg/kg Streptozotocin.SD rats with successful modeling were randomLy divided into model group,Coptis chinensis polysaccharide high-dose group(300 mg/kg/d),Coptis chinensis polysaccharide low-dose group(100 mg/kg/d)and losartan potassium group(30 mg/kg/d),with 10 rats in each group.Another 10 SD rats were selected as the normal group.Gavage,1time/d;after 8 weeks,the effects of Coptis chinensis polysaccharide on body weight,renal weight-weight ratio,FBG,BUN,Scr,24-hour urine protein,renal histopathological morphology,SOD,CAT,GSH-PX,MDA,IL-6,hs-CRP and TNF-? expression in DM rats were detected and protein expression of JAK2 and STAT3 in kidney was detected by WB.Results: Compared with the model group,the weight of Coptis chinensis polysaccharide group increased significantly,but the ratio of renal weight to body weight decreased significantly.At the same time,the FBG,BUN,Scr level and 24 h urine protein of the high and low dose Coptis chinensis polysaccharide group were significantly decreased,and the pathological morphology of renal tissue was significantly reduced.Coptis chinensis polysaccharide can also increase the activity of SOD,CAT and GSH-PX in kidney tissues,and reduce the content of MDA in kidney tissues and serum IL-6,hs-CRP and TNF-?.WB results showed that Coptis chinensis polysaccharide could down-regulate the expression of JAK2 and STAT3 proteins in renal tissues.Conclusion: Coptis chinensis polysaccharide can reduce kidney injury in DM rats,which is related to inhibiting oxidative stress and inflammatory response. |
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