| Purpose The rat model of middle cerebral artery ischemia-reperfusion injury was made by the method of thread supposition.The changes of motor and memory behavior,the volume of cerebral infarction and the expression of SYN,VEGF and GFAP proteins in relevant regions of the brain were observed after electroacupuncture at Baihui,Neiguan,Sanyinjiao and Shuigou points.Methods1 Molding and grouping The SD rat model of left middle cerebral artery ischemia-reperfusion injury was made by thread occlusion,and was screened according to the Neurobehavioral Evaluation method proposed by Zea Longa E.Randomly divided into four groups: sham operation group,model group,electroacupuncture group and electroacupuncture control group,60 in each group.The first two groups were not treated with electroacupuncture,and the latter two groups were intervened with electroacupuncture.Each group was further divided into three groups: 7 days,14 days and 21 days,20 in each group.2 Electroacupuncture intervention 24 hours after the successful establishment of the model,the electroacupuncture group and the electroacupuncture control group were treated with electroacupuncture at the main acupoint Shuigou,Baihui,bilateral Neiguan and Sanyinjiao.Acupuncture once a day in the morning,each time for 30 minutes,every 7 days for a course of treatment(acupuncture 6 days rest 1 day).Model group and sham operation group were not treated by conventional feeding.3 Behavior score and cerebral infarction volume Each group of experimental rats were evaluated by the behavior of viaduct cross,Barnes maze,roller falling time detection and so on.After TTC staining,the volume of cerebral infarction was calculated by image analysis software.4 Western blot detection of SYN,VEGF,GFAP protein expression The brain tissues of the experimental rats in each group were taken at three time points respectively.Western blot was used to detect the expression of SYN,VEGF and GFAP.5 Statistical analysis All continuous variables are expressed as mean ± standard deviation,and the comparison between multiple groups is performed by one-way ANOVA,then post hoc test.LSD method is used when the variance is the same,and Dunnett’s method is used when the variance is not the same.The statistical results were calculated by SPSS for windows 20.0.Results1.Elevated cross: The activity of experimental rats in model group and electroacupuncture group was significantly lower than that in sham operation group and electroacupuncture control group.The comparison found that the activity of electroacupuncture group at each time point was better than that in model group(p<0.01).2.Barnes memory maze experiment: The latency of model group and electroacupuncture group into the hidden area was significantly longer than that of sham operation group and electroacupuncture control group,and the latency of electroacupuncture group into the hidden area was significantly shorter than that of model group at all time points(p<0.01).The longer the duration of electroacupuncture was,the more obvious the shorter the incubation period was in electroacupuncture group(p<0.01).3.Falling time of roller: The movement function of limbs in sham operation group and electroacupuncture control group was not damaged,and the falling time of roller in electroacupuncture group was significantly longer than that in model group at each time point(p<0.01).4.TTC staining: There was not cerebral ischemia in sham operation group and electroacupuncture control group,but the infarct area in electroacupuncture group was significantly smaller than that in model group(p<0.01).5.The expression of SYN,VEGF and GFAP in the sham operation group and electroacupuncture control group was weak by Western blot,while that in the model group and electroacupuncture group increased gradually with the prolongation of treatment time,and electroacupuncture could enhance the expression of SYN and VEGF and inhibit the expression of GFAP during ischemia in the two groups,which had significant meaning compared with electroacupuncture at each time point 7-21 days after operation(p<0.01).Conclusions1.The results showed that electroacupuncture could improve the symptoms of neurological deficit caused by middle cerebral artery ischemia-reperfusion injury.2.Electroacupuncture can restore the volume of cerebral infarction.3.Electroacupuncture can reduce the volume of cerebral infarction in ischemic area.4.Electroacupuncture can promote the high expression of SYN and VEGF in cerebral infarction area,and regulate the over expression of GFAP.5.Electroacupuncture can protect the neurovascular unit and promote the recovery of nerve function through multi-ways. |
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