Study And Effects Of HUC-MSCs On Jurkat Cells

Objectives:Human umbilical cord mesenchymal stem cells(HUC-MSCs)are potential stromal cells which are regarded as the most feasible stem cell group in cell therapy.At present,MSCs have been becoming hotspots in the treatment of Systemic Lupus Erythematosus(SLE).With the development of microRNA in SLE pathogenesis,more and more evidence shows that there is a correlation between miR-181a and SLE pathogenesis.At the same time,current studies also show that SLE is closely related to T lymphocyte dysfunction.Jurkat cells are tumor cells of acute T-cell leukemia,which often represent human T lymphocytes in experimental studies.In this study,we will compare and analyze Jurkat cells co-cultured with HUC-MSCs and Jurkat cells cultured separately,so as to study and observe the effect of HUC-MSCs on Jurkat cells and explore its mechanism and lay a foundation for further study of the possible role and mechanism of HUC-MSCs on T lymphocytes of normal persons and SLE patients.Methods:Jurkat cells cultured alone and Jurkat cells co-cultured with HUC-MSCs at different time periods(24 hour,48 hour,72 hour,96 hour and one week after culture)were sampled.The gene and protein expression of OPN,B-catenin,NF-kB,TNF-alpha and the expression of microRNA-181a were detected by western-blot and Rt-PCR.SPSS19.0 was used for comparative analysis.All data were expressed by means and standard deviation(X±SD).The measured values of each group were compared and analyzed by variance analysis of random block design and the difference was statistically significant p-value<0.05.Results:Compared with Jurkat cells cultured alone,the relative expression of TNF-α,NF-kB,OPN and B-catenin genes and proteins in Jurkat cells co-cultured with HUC-MSCs at 24 hour,48 hour,72 hour,96 hour and one week decreased significantly,while the gene expression of microRNA181a increased significantly and the gene expression became more and more obvious with the time of co-culture.Conclusion:HUC-MSCs can affect Jurkat cell function by down-regulating TNF-α,NF-kB,OPN,B-catenin gene and protein expression and up-regulating miR-181a gene expression.This suggests that HUC-MSCs may affect T lymphocyte of SLE patients through the same way,thus exerting its therapeutic effect.HUC-MSCs also can inhibit the abnormal proliferation of Jurkat cells and the activation of SLE-related pathway proteins.However,Jurkat cells can not completely represent T lymphocytes of normal people and SLE patients after all.Therefore,further experiments are needed to verify our hypothesis.