The Preparation And Properties Research Of Sericin/Hydroxyapatite/Polycaprolactone As Composite Scaffold Material

The functional necrosis of individual tissues and organs often occur in Clinical medicine.Usually,transplantation of tissues and organs is used to treat damaged parts of the body.This method can help humans to treat certain diseases caused by tissue and organ diseases to a certain extent.However,the number of donors is limited,and the price is high induring the treatment process,which will produce corresponding immune rejection.Therefore,for repairing damaged tissues and organs of the body to exploring a biological tissue engineering material is a top priority.Sericin,hydroxyapatite,and polycaprolactone are all good biomaterials.In this paper,we studied the composites with different content of Sericin,hydroxyapatite,and polycaprolactone,The composites were prepared with the method of blending and freeze drying.The mechanical properties,appearance structure,physical properties,degradation properties and biosafety properties of the scaffold materials were investigated.And the possibility of the scaffold materials applied to tissue engineering was evaluated to lay a foundation for the application of the composite materials to tissue engineering medical materials.Composite material forming effect is good.the material is white cylindrical,the composite internal structure were spongy and fluffy porous.The scanning electron micrograph shows that,the pore diameter of the material decrease as the content of hydroxyapatite increases.Moreover,the porosity of the material is gradually reduced,but the porosity is higher than 50%.Meeting the requirements of tissue engineering scaffolds can provide favorable conditions for the value-added adhesion of cells.As the content of PCL increases,the water content of the scaffold material gradually decreases,but the change is small.The composite materials has relatively good water absorption ratios was 5:5,6:4,7:3,all of which are greater than 50%;The swelling ratios of the composite materials with different ratios are relatively small,meeting the requirements of tissue engineering scaffold materials.The results of scanning electron microscopy showed that the interior of the composite scaffold was irregular pore-like structure,and there was no obvious phase separation between sericin,hydroxyapatite and polycaprolactone.The absorption peak corresponding to the amide bond in the spectrum of the scaffold material did not shift significantly.The proportion of the composite materials had no significant effect on the secondary structure of the material,and the heat of the composite scaffold material increased with the increase of the content of polycaprolactone.The decomposition temperature is increased and the thermal properties are improved.The composites showed strong characteristic peaks at 2?=21.5° and 2?=23.9°,indicating that different ratios have little effect on the crystal structure formed by the interaction between the three materials.There was no significant difference in the structure of the scaffolds with different ratios.This is consistent with the FTIR results.The degradation and biocompatibility of sericin/hydroxyapatite/polycaprolactone composite scaffolds showed that the pH of the degradation materials of the scaffolds were between 7.4 and 8.0,and the acidity and alkalinity of the composites were stable during in vitro degradation.There is no excessive acidity and alkalinity,and the chemical properties is stable.The control composite scaffold 19% initial weights were reduced six weeks after degradation in vitro,with appropriate degradation rate.The test group composite scaffold material are rather 34%.The cells grew well in the extract of porous SS/HA/PCL composites,and the cell viability of different proportions of sericin/hydroxyapatite/polycaprolactone materials was higher than that of the negative control group.The novel composite scaffold material is non-toxic and has good biodegradability and cell compatibility.