Analyzing Plasma Biomarkers Of Autism Based On Proteomics And Metabolomics Methods

Autism is a group of heterogeneous neurodevelopmental disorders caused by genetic and environmental factors.It is characterized by early onset of abnormal social communication and restricted repetitive behaviors and interests.The etiology of autism is extremely complex.Pathological abnormalities in nervous system development,nerve projection,synaptic plasticity,postsynaptic potential,and neuroimmunity are might be maternal or autologous,genetic or environmental,direct or indirect manifestations of autism.The heterogeneity of autism is very high.Besides,it is difficult to have symptomatic drug treatment.At this stage,early intervention is mainly carried out to enable patients to integrate into social life.Early diagnosis of the disorder can create opportunities for early intervention and treatment.Screening for early biomarkers in infants and young children is of great significance for the classification and diagnosis of autistic people.The current mainstream diagnostic methods lack effective biomarkers that can be used for diagnostics purposes.The plasma is convenient for sampling and contains abundant substances and is suitable for screening diseases.This study used proteomics and metabolomics methods to analyze the blood plasma in autistic and healthy control 2-6 years' old children.The importance of the complement and coagulation cascades and the possible presence of extracellular matrices in autistic patients have been highlighted by individual independently labeled iTRAQ experiments,further deepening the understanding of the disease and the discovery of plasma biomarkers associated with autism.The differential proteins ADAMTSL4,AZGP1,and PLTP can be further studied as potential candidate proteins for autism plasma biomarkers.Through 124 autism and 97 control plasma non-targeted metabolomes,it has been found that a wide range of abnormalities in glycerophospholipid metabolism may have the potential to distinguish between autism and its subtypes.In addition,the pathways for bile acid synthesis and bile secretion in differential metabolites have certain credibility,but there are still many uncertainties in the identification of metabolites,which demand further analysis.Based on the differential proteins and metabolites found here,the pathological understanding of autism can be further deepened for the study of autism biomarkers.