Establishment And Formation Mechanism Of A Rat Model Of Liver Steatosis Induced By High Erucic Rapeseed Oil

The effects of high erucic acid rapeseed oil on rat liver fatty acid metabolism were studied in this thesis,and the mechanism by which erucic acid feeding on liver fatty acid metabolism was also investigated.SD rats were used to set up the rat model of liver steatosis induced by high erucic acid rapeseed oil,after fed with rapeseed oil for four weeks,the livers of the rats were taken out for physiological and biochemical analysis.Compared with the control group,liver index,body weight and serum TG level were increased significantly in the rats fed rapeseed oil,so as the hepatic TG,H₂O₂,ROS,MDA level,also,massive accumulation of microbubble fat droplets were observed in the hepatic cells.Compared with the control group,hepatic acyl-CoA oxidase activity of experimental group was significantly increased,while catalase activity was unaltered.The phosphorylation levels of AMPK?Thr172?and ACC?Ser79?of experimental group were significantly lower than those of the control group,while the phosphorylation level of p70S6K?Thr389?was significantly higher.The expression level of pex1,pex5,pex13,and pex14 in the livers of the experimental group was remarkably enhanced.The decreased phosphorylation level of AMPK would increase ACC activity,resulting in accumulation of cytosolic malonyl-CoA,thereby inhibiting the activity of carnitine palmitoyltransferase on the mitochondrial membrane,which eventually led to decrease in mitochondrial fatty acid?-oxidation and accumulation H₂O₂ and ROS that derived from peroxisomal fatty acids oxidation.In summary,excessive intake of rapeseed oil with high erucic acid led to hepatic fat and ROS accumulation,which may be a potential cause of metabolic diseases.