MiR-598 Inhibits Metastasis In Colorectal Cancer By Suppressing JAG1/Notch2 Pathway Stimulating EMT

Objective: To explore the role of miR-598 in colorectal cancer(CRC)and elucidate the mechanism.Methods: microarray-based strategy was applied to identify differentially expressed miRNAs in CRC by comparing miRNAs profile between CRC tissues and para-carcinoma tissues from CRC patients,qRT-PCR was used to testify the microarray.The effect of miR-598 on CRC cells metastasis was evaluated by Transwell migration assay.The effect of miR-598 on CRC cells EMT was determined by Western blot analysis and Immunofluerescence.Target bioinformatics software,miRanda,TargetScan and DIANA-microT,were applied to identify the target gene of miR-598,the prediction was validated by Western blot analysis and qRT-PCR.Transwell migration assay and Western blot was performed to identify the effect of JAG1 and Notch2.Results: miR-598 is found downregulated in both CRC tissues and cells,and our research indicates that miR-598 inhibits CRC cells metastasis and EMT.MiRanda,TargetScan and DIANA-microT,were applied to identify Jagged1(JAG1)as putative miR-598 target,the prediction were further reliably validated by Western blot analysis and qRT-PCR.In the study,we found JAG1 interacts with Notch2 in CRC cell lines.JAG1 and Notch2 have a promoting effect on CRC cells metastasis and EMT,and weaken the function of miR-598.Conclusion: miR-598 can inhibit metastasis in colorectal cancer by suppressing JAG1/Notch2 pathway stimulating EMT.