Microarray Study Of Gene Expression Of Chondrocyte In Leptin-induced Knee Joint Osteoarthritis

BACKGROUND: Microarray analysis has been widely used in gene detection.This technique was performed in gene expression profile of leptin-induced rat OA chondrocytes to explore the role of leptin on gene expression of the chondrocytes and regulation of apoptosis in rat with OA in the genetic level.METHOD:The experimental animals were divided into three groups: leptin-induced group,OA group,and blank control group.The gene expression profile of articular cartilage was analyzed by microarray and verified by quantitative reverse transcription polymerase chain reaction(RT-q PCR).The function of differential gene was evaluated by gene ontology(GO)and pathway analyses.RESULT:Leptin induced the destruction and degradation of articular cartilage,which could lead to the occurrence of OA.By analyzing the results of RNA hybridization between leptin-induced and blank control groups,there were 1857 genes in the two groups,among which 1197 genes were up-regulated and 660 genes were downregulated.Interleukin-1?(IL-1?),matrix metalloproteinases(MMPs,MMP-3,MMP13),and insulin-like growth factor binding protein 6(IFGBP6)were significantly up-regulated in the expression of OA-related genes.A new candidate gene,namely BCL2L11(BIM),was noted to up-regulate expression of OA-related genes,which might play an important role in OA progression.CONCLUSION:Leptin could impact on the progression of OA.It could promote the occurrence of OA by regulating the expression of multiple OA-related genes.A new candidate leptin-induced gene(BCL2L11)provides a theoretical evidence to elucidate the molecular mechanism of OA progression.It could also serve for the potential therapeutic target in treatment of OA.