Effect Of Sodium Hydrogen Sulfide On Oxidative Respiratory Chain Of Epidermal Cells Treated With Burn Serum And Its Mechanism

Objective To investigate the effect of Sodium hydrogen sulfide on the oxidative respiratory chain of epidermis cells treated with burn serum and verify that the ATP-sensitive K-channel(KATP channel)is a pathway that mediates the action of hydrogen sulfide on the epidermal cells.Methods The epidermis cells extracted from the back skin of neonatal rats were taken as the object of study.Normal serum and burn serum were prepared by taking normal SD rats and 30%?°burn SD ratundefineds main arterial blood.The epidermis cells of neonatal rats were divided into 5 groups:normal serum group,burn serum group,burn serum+NaHS group,burn serum+Gli group and burn serum+Gli+NaHS group.According to the requirements of each group,the complete medium of different reagents was prepared to interfere with the epidermal cells cultured in vitro,and the epidermal cells of each group were collected and the mitochondria were extracted.The activity of cytochrome C oxidase(CCO)was detected by spectrophotometer,and the respiratory control rate(RCR)of mitochondria was detected by oxygen electrode method.Results(1)The activity of cytochrome C oxidase in the epidermal cells of the burn serum group was significantly lower than that of the normal serum group(p<0.05);The activity of cytochrome C oxidase in the epidermal cells of the burn serum+Gli group was significantly lower than that of the burn serum group(p<0.05);The activity of cytochrome C oxidase in the epidermal cells of the NaHS group was significantly higher than that in the burn serum group(p<0.05);The activity of cytochrome C oxidase in the epidermal cells of burn serum+Gli+NaHS group was significantly lower than that of burn serum+NaHS group(p<0.05).(2)The mitochondrial respiratory control rate of epidermis cells in burn serum group was significantly lower than that in normal serum group(p<0.05);The mitochondrial respiratory control rate of epidermis cells in the burn serum group+Gli group was significantly lower than in the burn serum group(p<0.05);The mitochondrial respiratory control rate of epidermis cells in the burn serum group+NaHS group was significantly higher than in the burn serum group(p<0.05);The mitochondrial respiratory control rate of epidermis cells in burn serum+Gli+NaHS group was significantly lower than that in burn serum+NaHS group(p<0.05).Conclusions Hydrogen Sulfide can alleviate the effect of the burn injury on oxidative respiratory chain enzyme activity in mitochondria of epidermal cells;Hydrogen Sulfide can alleviates the effect of the burn injury on Mitochondrial Respiratory function of Epidermal cells;ATP-sensitive K~+channel is a pathway that mediates the pathophysiological effect of hydrogen sulfide on epidermal cells.