Expression And Significance Of NQO1 And HO-1 Proteins In T-cell Lymphoma

Objective To investigate the expression of NAD(P)H: quinone oxidoreductase 1(NQO1)and hemeoxygenase-1(HO-1)in T-cell lymphoma(TCL),and investigate the correlation between these two indicators and other clinicopathological parameters in TCL.Methods 1.Clinical data of 61 patients with TCL(TCL group)who were initially diagnosed in Gansu Provincial Hospital from December 2012 to May 2018 were analyzed retrospectively,the pathological types included 33 cases of extra-nodal NK/T-cell lymphoma,nasal type(ENKTL),8 ALK-negative anaplastic large cell lymphoma(ALK-negative,ALK-ALCL),6 cases with ALK-positive anaplastic large cells Lymphoma(ALK-positive,ALK + ALCL),7 cases with peripheral T-cell lymphoma(not otherwise specified,PTCL-NOS),4 vascular immunoblasts angioimmunoblastic lymphadenopathy(AITL),2 cases of enteropathy type T cell lymphoma(EATL)and 1 case with subcutaneous panniculitis like T cell lymphoma(SPTCL).20 cases of reactive hyperplastic lymphoid tissue were selected as control group.2.Immunohistochemical SP examination was performed to detect NQO1 and HO-1 expression levels in 61 TCL tissues(TCL group)and 20 lymph node reactive hyperplasia tissues(control group).Then the relationship between TCL clinical pathological parameters(pathological types,gender,age,lactate dehydrogenase level,clinical stage,B symptoms and bone marrow involvement)and expression of NQO1 and HO-1 were analyzed.Furthermore,the correlation between NQO1 and HO-1 expression in TCL were evaluated.Results The positive expression rates of NQO1 and HO-1 were significantly higher in TCL tissues than in lymph node reactive hyperplasia tissues(P<0.05).NQO1 expression was closely related with Ann-Arbor clinical stage and B symptoms(P<0.05);HO-1 expression was correlated with clinical stage,B symptoms and bone marrow invasion(P<0.05);NQO1 and HO-1 expression levels were not related to pathological types,gender,age and lactate dehydrogenase level(P>0.05);there was a correlation between NQO1 and HO-1 expression(r=0.264;P=0.040).Conclusion NQO1 and HO-1 are highly expressed in TCL,which may interact and contribute to the occurrence and development of TCL,and may provide new ideas for the etiology and therapy of TCL.