MiRNA-34a Promotes The Activity Of Cervical Cancer And Inhibits Its Migration And Invasion

[Background]Small RNA is a important member of non-coding RNAs.It is a kind of macromolecular compound that regulate gene expression at the post-transcriptional level.They are highly conserved small non-coding RNAs molecules that regulate various physiological and pathological processes including development,proliferation,differentiation,apoptosis,metastasis.According to the research,changes of the human mi RNAs may alter the physiological function of human,leading to the initiation and progression of cancer.mi RNA-34 a is a well-known mi RN As,Recent reports have demonstrated the involvement of mi RNA-34 a in cancer.However,we know little about the relationship between mi RN A-34 a and cervical cancer.O ur research aims to discuss whether mi RN A-34 a has a influence on the initiation and progression of cervical cancer and to reveal the mechanism by prediction of its target genes.[Methods] At first,PCR(real-time PCR)was used to detect the expression level of mi RN A-34 a in cervical carcinoma tissue samples and matched adjacent normal tissues samples.Then changed the expression level of mi RNA-34 a in He La cells use pri-mi RNA-34 a and Antago-mi RN A-34 a.MTT colorimetric assay,colony formation assay and migration and invasion assays was performed to detect the role of mi RN A-34 a in the activity,growth abilities,migration and invasion of He La cells.Subsequently,we chosed RIG-I as the target gene of mi RN A-34 a by bioinformatics.Western blot and real-time PC R were used to confirm the relationship between mi RN A-34 a and RIG-1.Fluorescent reporter experiment further confirmd that mi RN A-34 a regulated RIG-1 by targeting the 3'UTR region of RIG-1.Then,overexpress or knockdown RIG-I by Flag-RIG-1 and psh R-RIG-I and detect the function of RIG-I in activity,growth,migration and invasion of He La cells.Finally,the rescue experiments ensured that mi RN A-34 a affect activity,growth,migration and invasion of He La cells by regulate RIG-1.To analyze the detail mechanism of the regulation,Flow C ytometer were used to detect the effect of mi RNA-34 a or RIG-1 on apoptosis and cell cycle of He La cells.Then,Western blot and F luorescent reporter experiment were used to detect the level of autophagy.Finally,Western blot was used to detect its effect on EMT.[Results] In He La cells,overespression of mi RNA-34 a promoted the growth but inhibited the migration and invasion;on the contrary,the decrease of mi RN A-34 a inhibited the growth however promoted the migration and invasion.overespression of RIG-I promoted the growth but inhibited the migration and invasion;knockdown of RIG-I inhibited the growth however promoted the migration and invasion.Fluorescent reporter experiment,real-time PCR and Western blot were used to confirm that mi RN A-34 a directly target the 3 'UTR region of RIG-I and regulate the expression of RIG-I.Mechanism research showed that overespression of mi RN A-34 a and RIG-I could inhibit apoptosis,autophagy and EMT and accelerate cell cycle.The decrease of mi RNA-34 a and RIG-I made the contrary effect.[Conclusions] mi RNA-34 a could promote the growth but inhibit the migration and invasion of He La cells.mi RNA-34 a may achieve its goal by regulating its target gene RIG-I.Its mechanism is inhibiting apoptosis,autophagy and EMT and accelerating cell cycle.