The Expression Of MTA2 In Endometrial Carcinomas And The Correlation With Clinicopathological Features

Purpose: Metastasis-associated gene 2(MTA2)is reported to play as a promoter in tumor progression,but little is known about the role of MTA2 in endometrial cancers.The aim of the study was to explore the expression of MTA2 in endometrial cancer(EC)and its correlation with clinicopathological features.Methods:The GCBI database was used to analysis the MTA2 expression in majority cancers.Immunohistochemical staining of MTA2,P53,ER,PR and Ki-67 was performed to 119 EC tissues and 21 corresponding adjacent nonneoplastic endometria.And the correlation between MTA2 expression and clinicopathological characteristics was evaluated.The expression of MTA2 mRNA in EC cell lines with various differentiation levels was detected in vitro by RT-PCR.And the impact of MTA2 knockdown on Ishikawa and AN3 CA cells' proliferation was evaluated by CCK-8,clone formation and cell cycle assays.Results : We found that MTA2 overexpressed in endometrial carcinoma compared with the adjacent normal tissues(p < 0.001),and the expression level was related to tumor grade and ki-67 expression(p<0.05,p<0.05).In vitro,MTA2 mRNA expression is different in various differentiated EC cell lines.And knockdown MTA2 suppressed the proliferation of the Ishikawa and AN3 CA cells.Conclusions:These results suggested that MTA2 may act as an oncogene in EC,and it is a promising target for EC diagnosis and treatment.