| Background and Objective In spite of more and more studies on the relationship between nonalcoholic fatty liver disease and intestinal flora,the influence of NAFLD on certain specific intestinal bacteria and the relationship between intestinal microflora and bile acid metabolism have not been determined.The purpose of this study was to investigate the effects of NAFLD on the intestinal tract,including the effects of intestinal microflora,metabolites,intestinal permeability and so on.Methods Use the intestinal microbial metagenomics and metabonomics analysis of plasma,liver,colon,small intestine and feces to understand the intestinal microflora spectrum,metabolic profile changes and effects on intestinal barrier function of the rat model of NAFLD,.Results Analysis of intestinal microflora showed that NAFLD decreased the diversity of intestinal microflora.Gut microbiota such as Phascolarctobacterium?Blautia?Ruminococcaceae and so on in the NAFLD group significantly increased.The content of Lachnospiraceae was decreased.Compared with the control group,NAFLD group has not only the metabolic changes in plasma and liver,but also the metabolic changes of other tissues.The main changes were limited to lipid metabolism and bile acids.Combined with the analysis of correlation between intestinal microflora and bile acid,the content of colitis promoting strains such as Clostridium ? Desulfovibrio and Erysipelotrichaceae significantly increased,with Probiotics reduction.We speculate that such a change in flora may lead to elevated levels of secondary bile acids in the gut.The intestinal permeability of NAFLD group was significantly higher than that of control group.Conclusions NAFLD decreased the microbial diversity in the gut,and affected the species composition,distribution and abundance of microbial populations.The occurrence of NAFLD leads to the decrease of probiotics and influence the metabolism of bile acids.NAFLD can also affect the change of intestinal permeability. |
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