Biological Responses Of Human Bone Mesenchymal Stem Cells To Ti And TiZr Implant Materials

Background:Titanium-zirconium alloy?TiZr1317?is a new material used for biological implants.There are several studies on the effects of TiZr implants on the biological characteristics of human bone mesenchymal stem cells?hBMSCs?.Objectives:The purpose of this study was to investigate the biological responses of hBMSCs to implant holes affected by the physicochemical properties of oral implants?TiSLA,TiSLActive,TiZrSLA,and TiZrSLActive?.Materials and Methods:Grade 4 Ti and TiZr?13–17%Zr?substrates were modified by sandblasting and acid-etching?SLA?or hydrophilic sandblasting and acid-etching?SLActive?,resulting in four types of surface with complex microstructures corresponding to the commercially-available implants SLA?,Roxolid?SLA?,SLActive?,and Roxolid?SLActive??Institute Straumann AG,Basel,Switzerland?.Physicochemical properties were detected and the biological responses of hBMSCs were observed.Results:The surface contact angles of Ti_SLActive and TiZr_SLActive were 1.6 ⁰+0.3 ° and1.5 °+0.2 °,and the surface contact angles of Ti _SLALA and TiZr_SLA were 101.4+4.6 ° and96.20+3.0 °,respectively.SEM showed that the nano-protrusions on the surface of Ti_SLALA and TiZr_SLA were less or even absent,while the nano-protrusions on the surface of Ti_SLActive and TiZr_SLActive were relatively more;the namo-protrusions on the surface of Ti_SLActive surfaces were small and dense relatively,but the nano-protrusions of the TiZr_SLActive had large diameters and were dispersed relatively.The optical profiler and AFM showed that the surface roughness of Ti_SLActive and TiZr_SLActive was significantly higher than that of Ti_SLALA and TiZr_SLA?P<0.05?.Raman spectroscopy showed that only the amorphous TiO₂ was present on the surface of the Ti _SLA group,while the rutile TiO₂ characteristic peak was observed in the other three groups,but the lateral inhomogeneity was observed.After Raman shift 610cm^-1,the Raman spectra of four groups were similar.hBMSCs seeded on SLActive substrates exhibited better performance in terms of cell attachment,proliferation and osteoblastic differentiation than cells seeded on SLA.Conclusion:Because of their more suitable physicochemical properties,Ti _SLActive and TiZr_SLActive materials demonstrated more pronounced effects on the biological responses of hBMSCs compared with TiSLA and TiZrSLA.