Effect And Mechanism Of Probiotics On Mouse With Hyperuricemia Serum Uric Acid Level

Uric acid is the final product of purine metabolism in human body.The hyperuricemia may take place for intake of a large number of high purine foods,abnormal metabolism of purine,and the declining of renal function.Hyperuricemia can lead to the accumulation of urates in joints and surrounding tissues,leading to gout and other complications,such as chronic kidney disease,hypertension,and cardiovascular disease.Currently,there is no effective intervention method for controlling uric acid in blood over a long period of time.Part of exogenous purine compounds is metabolized by xanthine deaminase,which is secreted in the intestinal tract by E.coli to form uric acid.Then the uric acid is absorbed by the body.Two-thirds of the endogenous uric acid is excreted through the kidneys,and the remaining one-third is excreted from the intestines.Some of the excreted uric acid will be reabsorbed by the intestinal tract through the transporter protein.Therefore,increasing the decomposition and excretion of uric acid in the intestinal tract is a potential feasible way to reduce blood uric acid.Intestinal bacterium Lactobacillus and Pseudomonas capable decomposition uric acid into allantoin and urea.And the structure of gut microbiota is closely correlated with uric acid metabolism.In recent years the sequencing analysis patients fecal microbes flora with gout found that the structure of patients gut microbiota is changed dramatically compared with normal in home and abroad.In order to enhance the capacity of gut microbiota uric acid decomposition,the survey found there is a kind of fermented food Jiangshui in the north-west,and it can significant lower incidence of gout with the frequency increase of eating Jiangshui,so this study first screened Lactobacillus fermentium JL-3 belongs to the edible probiotics has strong uric acid degradation ability from Jiangshui.This paper mainly studied the effect of Lactobacillus fermentium JL-3 on the serum uric acid concentration,the excretion of uric acid,and the structure and function of gut microbiota in hyperuricemia mouse model.We combined oteracil potassium and uric acid to build hyperuricemia mouse model based on the method from literature.We give mouse more than 10~8 cfu Lactobacillus fermentium JL-3 by lavage every day,then we detect the change of mice serum,urine and feces uric acid concentration and the serum biochemical indexes related to renal function and inflammation.We also detect the 16S rRNA of mice fecal flora by Illumina Miseq sequencing.The results showed that in model mouse the serum and urine uric acid decreased significantly after the intervention of Lactobacillus fermentium JL-3,while the uric acid content in feces increased.The detection of inflammatory cytokines in mouse liver and kidney showed that JL-3 significantly reduced the concentration of IL-1?in liver,kidney and serum.The proportion of gut microbiota in hyperuricemia mouse is significantly increased in Bacteroidetes,while the proportion of Firmicutes and Proteobacteria is decreased.After the intervention of JL-3,the mouse gut microbiota structure is partially restored.When mice were only given oteracil potassium to increased serum uric acid,JL-3 did not reduce the uric acid concentration in the blood of mice.The results showed that JL-3 was planted into the intestinal tract of mice and maintained the structure of entire intestinal flora.Then the ability of the intestinal flora to degrade uric acid is improved.JL-3 has no significant inhibitory effect on the generation of endogenous uric acid,and mainly reduces the concentration of uric acid in blood by increasing the excretion of uric acid in the intestinal tract,and meanwhile reduces some inflammatory reactions caused by hyperuricemia.This study suggests that functional probiotics can reduce serum uric acid on a certain extent as dietary supplements.And it provides a new idea to control serum uric acid for a long time.