Regulatory Effect And Mechanism Of CD4+CD25+CD127low/Foxp3Treg Cells In Alveolar Echinocococosis

Objective To investigate the expression of Foxp3 in CD4+CD25+CD127lowTreg cells and to study the mechanism of CD4+CD25+CD127low/Foxp3 Treg cells in alveolar echinococcosis.Methods The mice were divid-ed into alveolar echinococcosis group and healthy control group.The percentage of CD4+CD25+CD127lowTreg cells and CD4+CD2 5+F oxp3 Treg cells and the expressions of Foxp3 in CD4+CD25+CD127lowTreg cells were detected by flow cytometry.The levels of IL-10 and TGF-?1 in serum were detected by ELISA.The relationship be-tween CD4+CD25+CD127low/Foxp3 Treg cells and lesion size was analyzed by Pearson Correlation Analysis.Results1.The percentage of CD4+CD25+CD127lowTreg cells in the healthy control group was 9.04 � 2.78,while it was14.91 �4.21 in the alveolar echinococcosis groups(P<0.004).2.The percentage of CD4+CD25+Foxp3 Treg cells in the healthy control group was 0.76 � 0.73 and 4.21 � 2.08 in the alveolar echinococcosis group(P<0.000).3.The con-ent of Foxp3 in CD4+CD25+CD127LOwTregcells was 70.7%in healthy control group and 67.7%in the alveolar echinococcosis group.4.The expression of CD4+CD25+CD127lowTreg cells in alveolar echinococcosis group was posi-tively correlated with the size of the lesions(r=0.894,P=0.01).And the expression of CD4+CD25+Foxp3 Treg cells in alveolar echinococcosis group was positively correlated with the size of the lesions(r=0.714,P=0.031).5.the level of IL-10 in the healthy control group was 533.82�55.82,while it was 1946.80�260.89 in the alveolar echinococcosis group(P<0.000).The level of TGF-?1 in the healthy control group was223.90�56.34,while it was 321.35�36.44 in the alveolar echinococcosis group(P<0.01).Conclusion 1.Echinococ-cus multilocularis infection can stimulate the proliferation of CD4+CD25+CD127low/Foxp3 Treg cells in mice,and CD4+CD25+CD127lowTreg cells express Foxp3 highly,which can be used as surface markers of Treg cells in alveo-lar echinococcosis;2.The expression of CD4+CD25+CD127low/Foxp3 Treg cells was positively correlated with the size of the lesions.Immunosuppressive cytokines(IIL-10,TGF-?1)secreted by Treg cells from the in-fected mouse were highly expressed,which indicated CD4+CD25+CD127low/Foxp3 Treg cells can promote the devel-opment of alveolar echinococcosis,and the mechanism may be related to the inhibition of the body's immune func-tion.