Bioinformatics Analysis And Functional Study Of LncRNA-ENST00000513396 In Colorectal Cancer

OBJECTIVE: Colorectal cancer(CRC)is one of the most common malignancies in the world,with an increase of approximately 1.3 million cases worldwide annually.The incidence and mortality of colorectal cancer in China are increasing year by year.Early diagnosis and treatment are the crucial way to improve the prognosis of patients with colorectal cancer.Long noncoding RNAs(lncRNAs)are a class of transcripts longer than 200 nucleotides with limited protein coding potential.Recently,many studies have revealed that lncRNAs were abundant in cells and might have multiple functions in a wide range of biological processes,such as proliferation,apoptosis,or cell migration of various tumors.However,the role of lncRNA in the development of colorectal cancer is not In-depth studied.METHODS: We applied Agilent microarray in tumoral tissues and paired paracancer tissues for detecting different expression of lncRNAs and mRNAs.Bioinformatics analysis was used to identify the relationship between lncRNAs and corresponding mRNAs and the potential function of the lncRNAs.ENST00000513396 was chosed for further functional study through constructing overexpression plasmid,cell invasion and cell migration.RESULTS: We found that 460 lncRNAs were significantly deregulated in cancer tissues,more importantly,they may participate in a variety of tumor-related signaling pathways through functional enrichment analysis with their co-expressed mRNAs,suggesting that these lncRNAs might play critical roles in tumorigenesis and development.Notably,we found lncRNA-ENST00000513396 was significantly upregulated in cancer tissues compared with paracancer tissues.The overexpression of ENST00000513396 promote tumor cell proliferation.Meanwhile ENST00000513396 could promote migration and invasion ability of colorectal cells.Conclusion: LncRNAs play an important role in the development of colorectal cancer.Differentially expressed lncRNAs are associated with multiple tumor signaling pathways.Overexpression of lncRNA-ENST00000513396 in colorectal cancer cells can promote tumor cell proliferation and increase the ability of cell invasion and migration,which make progress of tumor development.