LINC00910 Regulated The Expression Of LATS2 By Competitively Sponging MiR-372-5p To Promote The Malignant Progression Of Gastric Cancer

Objective: We are aimed to explore the expression level,biological function and mechanism of LINC00910 in gastric cancer(GC).Methods: Differential expression of LINC00910 was systematically evaluated in the Cancer Genome Atlas(TCGA)GC database.The 29 pairs of GC tissues and adjacent nontumor tissues were recruited from the Second Affiliated Hospital of Nanjing Medical University to verify the TCGA result by RT-PCR.The expression of LINC00910 in gastric cancer cell lines(MGC-803,SGC-7901,BGC-823 and MKN-28)and the human gastric epithelial cell(GES-1)were measured by RT-PCR.We knockdown the expression of LINC00910 to investigate the effects of LINC00910 in vivo.The biological functions were demonstrated by luciferase reporter assay and western blotting.Results: LINC00910 was highly expressed in GC tissues and cells(SGC-7901 and BGC-823).The expression of LINC00910 was associated with tumor differentiation.GC patients with high LINC00910 expression had poor prognosis.Knockdown the expression of LINC00910 inhibited the proliferation,invasion and migration of GC cells(SGC-7901 and BGC-823)in vivo.LINC00910 regulated the expression of LATS2 by competitively binding to mi R-372-5p.Conclusions: LINC00910 played the role of competing endogenous RNA(ce RNA)by sponging mi R-372-5p to regulate the expression of LATS2 and functioned as an oncogenic role in GC carcinogenesis and development.It formed LINC00910-mi R-372-5p-LATS2 axis which may be a potential therapeutic target and prognostic biomarker of GC.