Effects Of Icariin/Icaritin On The Expression Levels Of P-Tau,GSK-3? And PP2A In AD-like SH-SY5Y Cells

Objective: The neuroprotective effects of icariin/icarisin(ICA/ICT)on OA-induced AD-like cell models were investigated to explore its possible mechanism of action in the treatment of central nervous system degenerative diseases.Methods:Human neuroblastoma cells(SH-SY5Y)were cultured,and SH-SY5 Y cells were induced with different concentrations of okadaic acid(OA)to construct AD model cells.AD model cells were treated with different concentrations of ICA and ICT,and the cell activity changes after treatment were detected by CCK-8 method to select the drug dose and treatment time when the cell activity was the highest.The AD model cells were treated with the optimal drug dose and treatment time,and the protein was extracted.The relative expression levels of Tau,p-Tau,protein phosphatase 2A(PP2A)and glycogen synthase kinase-3?(GSK-3?)were detected by Western blot and ELISA before and after treatment.To evaluate the efficacy and possible mechanisms of ICA and ICT in the treatment of AD model cells after OA induction.Results: The growth state and proliferation activity of SH-SY5 Y cells after resuscitation culture were good.ICA and ICT had no obvious toxic effects on SH-SY5 Y cells in the range of 1-80 ?mol/L,and could promote cell proliferation.When the concentration of OA was 40nmol/L,the morphology of cells was significantly changed after induction of SH-SY5 Y cells,and the survival rate was higher.This concentration was used as the most suitable induction concentration.Treatment of 40 nmol/L OA-induced AD model cells with different concentrations of ICA and ICT showed that when the ICA concentration was 2.5?mol/L and the ICT concentration was 1 ?mol/L,the effect was best after 48 h of treatment.For the most suitable drug treatment concentration and duration of action.The results of ELISA and Western blot showed that the expression of p-Tau in SH-SY5 Y cells was significantly increased after induction(P<0.05),indicating that the AD model was successfully induced;after ICA/ICT treatment,p-Tau in AD model cells could be reduced(P<0.05).The expression level of PP2 A in ICA group and ICT group did not change significantly(P>0.05),while the expression level of GSK-3? decreased significantly(P<0.05).Conclusion: Both ICA and ICT may play a role in the treatment of AD-like cell models by down-regulating the expression levels of GSK-3? and p-Tau.