| In China,cervical cancer is one of the most common gynecological malignant tumors,as the main threatening for worldwide women.It is of great need to fully understand the potential occurrence mechanism of cervical cancer,thus developing the new therapeutic agents.HHLA2 is a new member of the newly discovered B7 family in recent years.It has been confirmed that it was overexpressed in some tumor tissues,but there is few report concerning HHLA2 expression and its clinical application.In this study,HHLA2 was found correlated to the clinical characteristics of cervical cancer and might become a new alternative target for the treatment in cervical cancer.Purpose:To study the correlation between HHLA2 expression and tumor infiltration T cells in human cervical and adjacent tissues,and their relationship with clinical pathological indexes.Methods:Forty cases of cervical cancer and 15 cases of adjacent tissue samples were selected from the Women and Children’s Health Institute in Shijiazhuang City from March 2015 to June 2018.All the Patients did not receive chemotherapy and or other targeted therapy before surgery.SP immunohistochemical staining was performed to analyze the HHLA2expression and infiltrating CD4~+T and CD8~+T cells lymphocytes in tumor tissues.All the experimental data were analyzed by the SPSS 19.0 statistical software package.Results:1.Expression of HHLA2 in human cervical cancer and adjacent tissuesImmunohistochemical staining results showed that HHLA2 was expressed in the cell membrane and cytoplasm in cervical cancer,and the positive expression rate was 97.5%.In adjacent tissues,HHLA2 is expressed on the surface of epithelial cells and macrophages,and the positive expression rate was only 6.7%,which was statistically significant compared with that on the tumor tissue(P<0.05).There was no statistical difference of HHLA2expression level in cervical cancer among the clinical features,including patient age(P=0.218),tumor size(P=0.433)and clinical stage(P=0.749),but there was a significant difference between pathological grade(P=0.002)and lymph node metastasis(P=0.030)(P<0.05).The higher the pathological grade,the higher the level of HHLA2 expression,and the level of HHLA2 was significantly higher in patients with lymph node metastasis than that in those without metastasis.2.Infiltration of CD4~+T cells in human cervical cancer and adjacent tissuesIn cervical cancer,67.5%of CD4~+T cells were highly infiltrated,which was statistically significant compared with 20%of the infiltration in adjacent tissues(P<0.05).There was no significant difference in the intensity of infiltration of CD4~+T cells between the patient’s age(P=0.577),tumor size(P=0.215),pathological grade(P=0.785),lymph node metastasis(P=0.768)and clinical stage(P=0.822).3 Correlation between the expression level of HHLA2 and the intensity of CD4~+T cell infiltration in cervical cancer tissuesThere was no correlation between the expression level of HHLA2(rs=0.159,P=0.326)and the intensity of infiltration of CD4~+T cells in cervical cancer tissues.4.Infiltration of CD8~+T cells in human cervical cancer and adjacent tissuesIn cervical cancer,50%of CD8~+T cells were highly infiltrated,which was statistically significant compared with 6.7%of the infiltration in adjacent tissues(P<0.05).There was no significant difference in the intensity of infiltration of CD8~+T cells in cervical cancer and among the clinical features,including the patient’s age(P=0.113),tumor size(P=0.698),pathological grade(P=0.749),lymph node metastasis(P=0.490),and clinical stage(P=0.114).5 Correlation between the expression level of HHLA2 and the degree of CD8~+T cell infiltration in cervical cancer tissuesThere was a negative correlation between the expression level of HHLA2and the intensity of infiltration of CD8~+T cells in cervical cancer tissues(rs=-0.354,P=0.025,P<0.05).Conclusion:1.HHLA2 was abnormally highly expressed in human cervical cancer tissues,and its expression level was significantly associated with pathological grade and lymph node metastasis.HHLA2 expression might be functionally important in tumor progression and metastasis in cervical cancer.2.The expression of HHLA2 in cervical cancer was negatively correlated with the intensity of infiltration of CD8~+T lymphocytes,suggesting that HHLA2 may play an important role in suppressing immune surveillance of cervical cancer. |
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