MTA-induced Notch Activation Enhance HDPCs Proliferation By Inhibiting Autophagic Flux

MTA(Mineral Mrioxide Aggregate)is a new type of calcium silicate-based pulp cap materials,which has been widely used in dental clinical field because of its good biocompatibility,antibacterial and capping effect since the 1990 s.Notch signaling is a highly-conserved signal pathway which plays a central role in regulating cell proliferation,differentiation,and cell fate decisions.Recent studies have shown that Notch signaling plays an important role in the growth and differentiation of hDPSCs.The activation of Notch signaling pathway promotes cell proliferation while inhibits differentiation in hDPSCs.Autophagy is also a highly-conserved signal pathway in evolution which serves as one of the mechanisms of intracellular protein degradation,and plays an important role in regulating cell proliferation,differentiation,apoptosis,and immunity through the paraffin with degradation of proteins or organelles to form autophagosomes,and fused with lysosomes to form autophagosomes.Autophagy plays an important role in regulating cell growth and differentiation as one of the intracellular protein degradation "machines".Both of the Notch signaling and autophagy are important for cell proliferation and differentiation.The aim of this paper is to clarified the relationship between Notch signaling and autophagy in MTA promoting the growth and differentiation in hDPCs.Autophagy flux was significantly inhibited by MTA extracts,causing Notch degradation arrest.This resulted in promotion of cell proliferation and inhibition of differentiation during the logarithmic phase of cell growth.This research exploreed the molecular mechanisms of MTA-induced promoting the growth and differentiation of dental pulp cells activated b y the Notch signaling pathway.Our jobs revealed the relationship between autophagy and Notch signal pathway involving in cell growth and differentiation of dental pulp cells which is meaningful for guiding the clinical practice in using MTA correctly and also has very important practical significance to the development of pulp capping materials.