| ObjectiveMinimally Invasive Endodontics(MIE)is designed to apply minimally invasive procedures to every stage of endodontic treatment,including: diagnosis and treatment plan,access design,instruments,and apical surgery to to preserve the hard tissue of teeth to the maximum extent and increase the retention rate of the tooth.Compared with traditional access cavity,whether the dentin protective channel guided by the root canal can affect the extramedullary capacity of the pulp cavity and the root canal system,the removal of the debris,the necrosis and the cleaning capacity of the bacteria in the root canal are the hot spots at present at home and abroad.This experiment compared the effect of minimally invasive endodontic treatment with “truss” access cavity and traditional endodontic treatment of bacteria in the root canal,and provided effective experimental data for the wide application of minimally invasive endodontic treatment.MethodsAfter autoclaving all the isolated teeth,the Enterococcus faecalis suspension freshly prepared from bovine brain heart infusion(BHI)was inoculated on the 1st,7th and 14 th day respectively,and the culture medium was changed every other day.The temperature was at 37 °C in anaerobic culture.The model of Enterococcus faecalis infection was established for 3 weeks.One sample was randomly selected after each inoculation.After opening the medullary cavity,10# K file was licked in the root canal for 10 s,and dentin debris was taken to confirm that Enterococcus faecalis had invaded the teeth.In vitro double premolars to be collected were randomly divided into the Minimally Invasive Endodontics group and the Traditional Endodontic Cavity group.The MIE groups underwent “Truss” Endodontic Cavities under the guidance of CBCT.The TEC groups were in traditional way to establish a straight path.The MIE group and TEC group used ProTaper Gold mechanical preparation to F2,according to three different methods of chemical preparation were devided into 3 subgroups: Group A: TREC + 1% sodium hypochlorite,Group B: TREC + 1% sodium hypochlorite + Er:YAG laser,group C: TREC + 1% sodium hypochlorite + ultrasonic irrigation,group D: TEC + 1% sodium hypochlorite,group E: TEC + 1% sodium hypochlorite + Er:YAG laser,group F: TEC +1% sodium hypochlorite + ultrasonic irrigation,then the excised teeth are cut into three parts: the crown,the buccal root and the palate root.The dentin debris is taken with the 30#K file,and colony was counted on agar medium after 24 hours,and statistical analysis was performed in parallel.ResultsThe SAS software was used to statistically analyze the number of colonies in all three parts of the sample.The MIE group and the TEC group treated with 1% sodium hypochlorite regardless of different Endodontic Cavities,different tooth parts,the results of the interaction between the two groups were statistically significant(P < 0.05).There was a statistical difference between the laser group,the ultrasound group and the control group,and the difference was statistically significant(P<0.05).However,there was no statistically significant difference between the ultrasound group and the laser group(P>0.05).ConclusionChemical irrigation alone can not effectively remove the infection under the dentine truss and the pulp angle to the level of root canal cleaning under the traditional open vision.The equipment such as ultrasound or laser can help the chemical preparation greatly improve the situation and eliminate.The residual bacteria in the root canal can reach the same level of conventional treatment,and the results of laser irrigation are slightly better than those of ultrasonic irrigation.Under the premise of preserving the hard tissue of the tooth to enhance the fracture resistance of the tooth,the TREC combined with laser preparation or ultrasonic irrigation can achieve similar disinfection level of traditional endodontic treatment. |
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