The Expression And Its Clinical Significance Of CDK12 In Prostate Cancer

ObjectiveProstate cancer(PCa)is a common malignant tumor in male genitourinary system,and its incidence is increasing year by year in China.As a recognized cancer-related gene,cyclin-dependent kinase 12(CDK12)plays an important role in the occurrence and development of malignant tumors such as ovarian cancer,breast cancer and PCa.The expression of CDK12 in PCa has not been reported in China.In this study,we investigated the expression of CDK12 in PCa and benign prostatic hyperplasia(BPH)tissues and analyzed the correlation between the expression of CDK12 and the clinicopathological characteristics of PCa patients.MethodsSelecting 30 PCa tissue specimens and related clinical and pathological data.At the same time,20 BPH specimens were collected.The location and expression of CDK12 was detected by immunehistochemical(IHC)method,and the relationship between its expression and clinicopathological features of PCa patients was analyzed.The expression of CDK12 was compared between PCa and BPH using non-parametric test(Mann-Whitney U Test)of two independent samples.The correlation between CDK12 expression and clinicopathological characteristics of PCa patients was analyzed by Spearman rank correlation analysis.P < 0.05 was statistically significant.ResultsThe results of IHC showed that CDK12 was mainly located in the nucleus of prostate gland,and CDK12 was expressed in both PCa and BPH.The positive expression rate of CDK12 in PCa was significantly higher than that in BPH(p < 0.05).Spearman rank correlation analysis showed that there was no significant correlation between age,prostate specific antigen(PSA)level and CDK12 expression(p > 0.05),but the expression level of CDK12 was positively correlated with tumor stage and Gleason score(p < 0.05).ConclusionThe positive rate of CDK12 expression in PCa tissues was significantly higher than that in BPH tissues,and the higher the staging and Gleason score of prostate cancer,the higher the expression of CDK12,which suggesting that the overexpression of CDK12 may play an important role in the development of PCa,and the expression level of CDK12 is positively correlated with the degree of malignancy of PCa.