| Objective:To investigate the expression of guinea pig capsules on uterine smooth muscle cells(VSMC)phenotype markers a-smooth muscle actin(?-SMA)and proliferating cellnuclear antigen(PCNA)phenotypes in intrauterine device(IUD)model.Effects andeffects on the concentrations of thromboxane B2(TXB2)and6-prostaglandine(6-keto-PGF1?)in peripheral blood of rats.Method:Wistar female rats were randomly divided into normal group(n=16),model controlgroup(n=18),Guizhi Fuling capsule group(n=18)and aminocaproic acid group(n=17).4groups were given normal feeding and no treatment.After IUD modeling,0.9%normal saline,aminocaproic acid solution and cassia twig extract were applied.SPimmunohistochemical method was used to detect endometrial VSMC of each group.The expression of phenotype markers ?-SMA and PCNA was determined by ELISAto determine the concentrations of TXB2 and 6-keto-PGF1? in each group.Result:(1)The positive expression rate of VSMC phenotypic marker ?-SMA in the model group was significantly lower than that in the normal group(P < 0.05).The positive rate of ?-SMA expression in the endometrium of the model group was significantly higher than that in the control group(P < 0.05).The positive expression rate of?-SMA in Guizhi Fuling capsule group was significantly higher than that in model group(P > 0.05),but close to that in normal group(P > 0.05).The expression of?-SMA in amino-caproic acid tablet group was significantly higher than that in Guizhi Fuling capsule group(P < 0.01).The positive expression rate of ?-SMA was(50.89 ±9.41)% in normal group,(26.93 ±6.80)% in model control group,(48.92±6.80)% in Guizhi Fuling capsule group and(34.63 ±7.26)% in amino caproic acid tablet group.It was found that the positive expression rate of ?-SMA in endometrium of normal group and Guizhi Fuling capsule group was significantly higher than that of the other two groups.(2)The positive expression rate of proliferating cell nuclear antigen(PCNA)of endometrial VSMC in the model group was significantly higher than that in the normal group,and the expression of PCNA in each group was significantly decreased after treatment.Among them,the expression of PCNA in Guizhi Fuling capsule group was significantly lower than that in amino caproic acid tablet group(P < 0.05).The positive expression rate of PCNA was(25.66 ±7.24)% in the normal group,(61.26 ±9.98)% in the model control group,(28.36 ±9.17)% in the Guizhi Fuling capsule group,(50.23 ±8.71)% in the amino-caproic acid tablet group,and(28.36±9.17)% in the Guizhi Fuling capsule group.The positive expression rate of PCNA in endometrium of normal group and Guizhi Fuling capsule group was significantly lower than that of the other two groups.(3)Compared with the control group and Guizhi Fuling capsule group,the levels of6-keto-PGF1 ? and serum TXB2 in the model control group were significantly different,and those in the model control group were significantly different from those in the amino-caproic acid tablet group(P < 0.01).The serum TXB2 concentration was(445.86 ±24.43)ng/L in the normal group,(448.11 ±9.63)ng/L in the Guizhi Fuling capsule group,(508.78 ±12.42)ng/L in the model control group,and(498.11 ±13.63)ng/L in the amino caproic acid tablet group.Compared with normal group,amino acid group,Guizhi Fuling capsule group,model control group was significantly higher,while Guizhi Fuling group was close to normal group.The serum concentration of 6-PGF1? was(23.17 ±1.93)ng/L in normal group,(18.09±0.93)in model control group,(22.70 ±1.61)ng/L in Guizhi Fuling capsule group,and(20.70 ±1.41)ng/L,in amino-caproic acid tablet group.In the model control group,compared with the normal group,amino-caproic acid group,Guizhi Fuling capsule group was significantly lower.(4)Compared with the four groups,the Guizhi Fuling capsule group was the most close to the normal group.By comparing the correlation between VSCM phenotypic marker ?-SMA,PCNA and TXB2,6-keto-PGF1? in normal group,model group,Guizhi Poria group and amino caproic acid tablet group,the correlation coefficient r was 0.It means there is no correlation.Conclusion:1.Guizhi Fuling can effectively regulate the expression of ?-SMA,PCNA in the uterus of model rats,increase the expression of ?-SMA and decrease the expression of PCNA.2.Guizhi Fuling can effectively regulate the change of serum TXB2,6-keto-PGF1 ?concentration,increase the concentration of 6-keto-PGF1 ? by decreasing serum TXB2,regulate hemorheology,and improve the blood stasis state of rat uterus.3.The mechanism of Guizhi Fuling can reduce abnormal uterine bleeding in IUD rat model is related to the regulation of phenotypic protein expression in uterine smooth muscle cells and the regulation of hemorheology. |
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