Study On Extraction And Purification Process Of Stachyose In Stachys Sieboldii Miq

This paper uses plants of the genus stachyera Stachys sieboldii Miq as raw material,the purification process and activated carbon chromatography refined stachose was studied,the antibacterial activity of the Stachys sieboldii Miq is also studied.Extraction of total sugar from Stachys sieboldii Miq by ethanol reflux extraction,and use the response surface method to design experiments,optimize the extracyion process conditions.The effects of single factor ethanol concentration,solid-liquid ratio,extraction time,extracyion temperature on the extraction rate of total sugar from the Stachys sieboldii Miq were studied separately.Based on the single factor experiment results,optimum conditions for obtaining total sugar from Stachys sieboldii Miq by response surface methododogy:the ethanol concentration is 15.70%,the ratio of material to liquid is1:10.28(g/mL),the extracyion temperature is 65.06~0C,the extracyion time is 81.80min,under this condition,the extraction rate of total sugar from Stachys sieboldii Miq is 63.66%,fit to the height of the model.Static decolorization test using macroporous adsorption resin,by determination of the decolorization rate,stachyose retention rate and protein clearance rate of stachyose from Stachys sieboldii Miq,preliminary selection of resin AB-8 for purifying stachyose from 9 kinds of macroporous adsorption resins of different polarity and type,the resin has better purification effect on the stachyose,determined as the best purification resin.The optimum process conditions for the purification of AB-8 macroporous adsorptin resin are:temperature 30?,extract concentration 0.8g/mL,pH5,the aspect ratio is 1:4,the flow rate is2BV/h,under this optimal condition,the protein clearance rate was 58.64%,the stachyose retention rate was 82.51%,color removal rate was 75.34%,under this condotion,the purity of the Stachys sieboldii Miq stachyose is 73.09%.Purification of stachyose by activated carbon column chromatography,gradient elution with different concentrations of ethanol eluent,stachyose is concentrated in 15%ethanol eluent,HPLC results show that the purity of activated carbon refined stachyose reaches 87.01%.The study on the antibacterial activity of stachyose,the purified stachyose with the best process has a certain antibacterial effect on Staphylococcus aereus and Escherichia coli.