BDNF-induced Neuroprotection And Mechanism In Retinal Ischemia-Reperfusion Model

Retinal ischemia is a common cause of visual impairment and blindness.We use a chronic high IOP model--rats' retinal ischemia reperfusion(I/R),and first we had counted the axon numbers of the rats at different time points.We found that the axon densities and the total axon numbers were decreased obviously after six weeks.We also detected the visual function in the model and we found the rats loss their visual function seriously before the axons had lost.After that,we used western blotting to detect the quantitative change of Kinesin,Dynein and Tubulin.It showed that in the optic nerves,Kinesin and Dynein decreased significantly.The main methods to cure retinal ischemia has no obvious effects.We try to cure I/R model by brain-derived neurotrophic factor(BDNF),and we finally found that BDNF could improve the rats' visual function.And at the same time,we found there were higher levels of Kinesin and Dynein contents in the optic nerves after we injected BDNF into the I/R eyes than the I/R ones.So,it suggests that BDNF can also improve the axon transportation of I/R optic nerves.