Extraction And Separation And Biological Activity Of Ferulic Acid From Ligusticum Chuanxiong Hort By Deep Eutectic Solvents And Microwave

Chuanxiong Rhizoma,is a traditional staple of commonly used Chinese traditional medicine,with promoting blood circulation of Qi,dispelling wind and relieving pain,is an important and commonly drug for applied in the treatmenting of angiocardiopathy and gynecological disease.Ferulic acid is the major ingredient of organic acids in the L.chuanxiong,and it is also the biomarker of L.chuanxiong in the Chinese Pharmacopoeia.Several studies have demonstrated that ferulic acid exhibits a number of biological functions,such as antioxidation,anti-hyperlipidemia,anti-cancer and preventing cardiovascular diseases.At present,the demand of safe medicinal components is increasing with year,but the traditional extraction and separation methods in the industry have many problems such as low extraction efficiency,toxic solvent,complicated operation,and severe environmental hazard.Therefore to establish a safe and effective extraction and purification method for ferulic acid from L.chuanxiong is significant for the effective utilization and application of L.chuanxiong in new drug development,food and other industries.In this study,the novel and green natural deep eutectic solvents（NADES）were used as extraction solvent,the extraction and purification condition of ferulic acid from L.chuanxiong were investigated.Furthermore,the in vitro activities of ferulic acid from L.chuanxiong were evaluated by antioxidant and antimicrobial mothods.And the main results of research were as follows:1.The yields of ferulic acid were adopted as indexes,the optimum components of NADES were initially screened from 20 kinds of NADES,and the influence of NADES components ratios,water content on the yields were investigated.The results showed that the NADES composed of 1:1（mol/mol）of choline chloride/1,2-propanediol and 30%of water was proved the optimum NADES for extraction ferulic acid.On the basis of the single factor experiment results,the Box-Behnken design（BBD）combined with RSM was used to optimize the optimal extraction conditions of ferulic acid from L.chuanxiong.The optimum conditions were extraction time of 20 min,extraction temperature of 68°C,and solvent-to-solid ratio of 30:1 mL/g.The optimal yield was 2.32 mg/g（0.51%RSD）,which resided within the 95%prediction interval of 2.39 mg/g.It implied the model was reliable and applicable.2.Using macroporous resin,ferulic acid was purified in the NADES extract.The NKA-9 resin was chosen as the suitable resin for further research among the five kinds of resins.And the optimum conditions of NKA-9 resin were as follows:2 times diluents of initial extract solution,the loaded volume of 220 mL,adsorption time about 3.5 hours,50%ethanol of elution solvent,240 mL of elution volume,and 1.0 mL/min of flow rate.By this condition,the recovery ratio of ferulic acid was 86.95%,and the content of ferulic acid was 7.03%.3.The scavenging ability of DPPH radical,ABTS radical and hydroxyl radical by unpurified and purified ferulic acid were investigated.At the same time,the antimicrobial activity of unpurified and purified ferulic acid from L.chuanxiong were determined by Filter paper disc method to Staphyloccocus aureus,Bacillus subtilis and Escherichia coli.The results showed that unpurified and purified samples exhibited different scavenging rate of free radical.Compared with unpurified sample,the IC₅₀ values of purified sample to DPPH radical,ABTS radical and hydroxyl radical decreased 1.62μg/mL、1.46μg/mL and 9.81μg/mL,and the max scavenging rate increased 6%,1%,26%,respectively.In addition,both of purified and unpurified samples indicatived different intensity of inhibited effect for Staphyloccocus aureus,Bacillus subtilis and Escherichia coli,and the order was Staphyloccocus aureus>Escherichia coli>Bacillus subtilis.Those results suggested that the purified ferulic acid from L.chuanxiong displayed stronger in vitro activities than unpurified...