| Objective: To observe the changes of free radicals in hypothalamus of fatigue model rats and the mechanism of action of Mongolian warm acupuncture(MWA).Method:Fifty male SD rats(weighing 160-180 g)were randomly divided into five groups: normal group,normal + Mongolian warm acupuncture(NMWA)group,model group,model + Mongolian warm acupuncture(MMWA),model + moxibustion(MM)group,with 10 rats in each group.Three days before the establishment of the model,20 minutes of adaptive swimming each time,followed by weight-bearing(5% weight every three days)swimming,a total of 21 days.During swimming,if the rats do not float,they are driven away with sticks to keep them in motion.Failure to swim out of the water within 10 seconds is regarded as exhaustion.Remove quickly and wipe the towel to rest in the cage.Normal group and NMWA group did not make models.The MMWA group was treated with Mongolian medicine warm needle intervention at Dinghui point and Xinxue point alternately every other day.The MMWA group was treated with warm needle after exhaustive swimming.The MM group was treated with Moxibustion on both sides of Zusanli every other day,while the normal group and the model group were not treated.The general state was observed during the experiment.The standing times in 5 minutes,the time to find the platform in Morris water maze experiment,the number of times to swim across the platform and the fixed time of tail suspension experiment were compared before and after the experiment.While observing its behavior during the experiment,the hypothalamic free radicals were detected by the decapitation sampling method after 21 days.to explore the influence of the content of SOD,MDA and GSH-Px in the hypothalamic free radicals of the fatigue model rats and the action mechanism of MWA.Results:There was no significant difference in the general state of rats in each group at the early stage of model establishment.With the time of modeling,the rats in the model group showed obvious decrease in food intake and water consumption,no significant increase in body weight,dim hair color,poor mental health,less activity and irritability.The general state of MMWA group and MM group was compared with that of model group.1.Body weight: There was no significant difference in body weight between the groups before the model establishment(P>0.05).Compared with the control group,the model group,the MMWA group,and the MM group had significant differences(P< 0.05 or P<0.01);compared with the model group,there was a significant difference between the NMWA group and the MMWA group(P<0.05),and there was a significant difference between the MM group(P<0.05).2.Exhaustion time: Compared with the model group,there was a significant difference between the MMWA group(P<0.01),and there was a significant difference between the MM group(P<0.05).3.The suspension tail did not move time: There was no significant difference in body weight between the groups before the model establishment(P>0.05).After modeling,there was a significant difference between the model group and the control group(P<0.05).Compared with the model group,the NMWA group,MMWA group was significantly different(P<0.05 or P<0.01);4.Times of swimming across the platform andfinding the incubation period of the platform: Compared with the control group,the model group had significant difference(P<0.05).Compared with the model group,there were significant differences between the NMWA group,the MMWA group,and the MM group(P<0.05 or P<0.01).5.Open-field experimental standing times: There was no significant difference between the groups before modeling(P>0.05).After modeling,compared with the control group,the model group had significant difference(P<0.05),Compared with the model group,there were significant differences between the NMWA group,the MMWA group,and the MM group(P<0.05 or P<0.01).6.MDA content: compared with the control group,the MDA content of the model group increased significantly(P<0.05).Compared with the model group,the MDA content in the MMWA group and the MM group was significantly lower(P<0.05);SOD activity: Compared with the control group,the SOD activity of the model group was significantly lower(P<0.01).Compared with the model group,the SOD activity of the MMWA group and the MM group was significantly increased(P<0.05);GSH-PX activity:Compared with the control group,the GSH-PX activity in the model group was significantly lower(P<0.01);compared with the model group,the activity of GSH-PX in the the MMWA group,the MM group was significantly increased(P<0.05).Conclusion: 1.Mongolian warm acupuncture(MWA)significantly improved the mental state and behavioral ability of the fatigue model rats.2.MWA therapy has the function of regulating the hypothalamic free radicals in fatigue model rats,which plays an anti-fatigue role by regulating the neuro-endocrine regulation.3.MWA therapy plays a role in regulating the nervous system,endocrine system and immune system through acupuncture and warm stimulation.4.MWA therapy has the functions of regulating the dysfunction of Heyi Badagan,improving blood circulation,improving the body’s immune function,inducing mobilization and enhancing antiviral ability.MWA has the less acupoint,simple,safe,no side effects,multiple pathways,comprehensive treatment and other characteristics. |
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