Serum Vitamin D Level And The Gene Polymorphism Of Vitamin D Receptor Fok I In Children With Kawasaki Disease

ObjectiveTo study the correlation of serum 25-hydroxyvitamin D₃[25-?OH?D₃]level and gene polymorphism of vitamin D receptor?VDR?Fok I in children with Kawasaki disease?KD?.MethodWe collected clinical data of 136 children with KD admitted to the Children's Medical Center,Affiliated Hospital of Qingdao University from Jan.1^st,2017 to Dec.31^st,2018.According to the presence or absence of coronary artery lesion?CAL?,these children were classified into CAL group?50 children?and non-CAL?NCAL?group?86children?;According to the efficiency of intravenous immunoglobulin?IVIG?,these children were classified into IVIG-sensitive group?108 children?and no-IVIG-response group?28 children?.The control group includes 76 health children.The serum level of25-hydroxy vitamin D was detected by electrochemiluminescence,while the polymorphism of VDR Fok I gene locus was detected by Sanger sequencing.Results1.KD group has significantlylower serum levels of 25-?OH?D₃ than the healthy control group?t=18.90,P<0.05?.There was no significant difference in serum 25-?OH?D₃ level between CAL group and NCAL group?t=0.13,P>0.05?;There was no significant difference in serum 25-?OH?D₃ level between different levels of coronary artery injury?F=1.97,P>0.05?.No-IVIG-response groupgroups had significantly lower serum levels of 25-?OH?D₃ than the IVIG-sensitive group?t=2.33,P<0.05?.2.There are three genotypes at the T/C locus of VDR Fok I gene,including wild-type TT,mutant TC and CC.The distribution of the three genotypes has no difference in the KD and the control group?X²=2.08,P>0.05?;The distribution frequency of T and C allele also has no difference in two groups?X²=1.74,P>0.05?.3.TT,CT and CC genotypes of VDR Fok I gene at T/C locus showed no significant difference between CAL group and NCAL goup?X²=1.85,P>0.05?.There was no difference of allele frequency of allele T and C in the two groups?X²=1.78,P>0.05?.4.The distribution frequency of TT genotype of VDR Fok I gene was significantly higher in no-IVIG-response group than IVIG-sensitive group(?X²=3.97,P<0.05?;The distribution frequency of T allele at T/C locusof VDR Fok I gene was significantly higher in no-IVIG-response group than IVIG-sensitive group?X²=5.15,P<0.05?Conclusion1.Vitamin D levels may be related to the pathogenesis of Kawasaki diseaseand no response to treatment increases,but may not related to the possibility of CAL and degree of coronary artery injury.2.VDR Fok I gene polymorphism may not be related to the pathogenesis of Kawasaki disease and the possibility of CAL.TT genotype and T allele may be high risk factors for no-IVIG-response.