Crrelation Between Vitamin D Veceptor Vene Polymorphism And Prostate Cancer Risk

ObjectivesThe purpose of this study was to analyze the correlation between vitamin D receptor gene polymorphism,including FokI and BsmI,and the risk of prostate cancer,and to explore the correlation between VDR gene polymorphism and the development and prognosis of prostate cancer.MethodsThis experiment through the case-control study method,blood samples from122 patients with prostate cancer and the control group,150 cases of healthy check-up crowd blood specimen,application of restriction fragment length polymorphism polymerase chain reaction(PCR-RFLP),a system for VDR receptor gene polymorphism genotyping FokI,BsmI sites),determination of allele frequency and genotype frequency,and logical analysis for single factor and multiple factors to study different alleles and genotypes and the correlation of men with prostate cancer risk,in addition,The association of genotype frequency with stage,differentiation,Gleason score,metastasis and prognosis of prostate cancer was analyzed by using contingency table analysis.ResultsCompared with the control group,the genotype frequency and allele frequency of FokI loci of VDR gene polymorphism were statistically different(genotype:?~2=14.208,P<0.05;Allele:?~2=13.815,P<0.05).Univariate regression analysis showed that ff genotype in Fok I gene polymorphism was significantly correlated with the risk of prostate cancer(P=0.040,OR=1.881,95%CI=1.029~3.437).Prostate cancer patients with Ff+Ff genotypes carrying at least one f locus also had a significantly increased risk(P<0.001,OR=2.659,95%CI=1.571~4.502).The f allele was significantly associated with the risk of prostatecancer(P<0.001,OR=1.452,95%CI=1.192~1.769).Afteradjustingfor confounders such as age,smoking history and drinking history,multivariate regression analysis was performed,and the results showed that the ff genotype of the FokI locus of VDR gene polymorphism was significantly correlated with the risk of prostate cancer(P=0.024,AOR=2.065,95%CI=1.099-3.880).Ff and Ff genotypes with at least one f allele werealsosignificantlyassociatedwithprostatecancerrisk(P<0.001,AOR=2.811,95%CI=1.642~4.814).Compared with the control group,the genotype frequency and allele frequency of BsmI loci of VDR gene polymorphism were statistically different(genotype:?~2=7.154,P=0.028;Allele:?~2=5.932,P<0.015).Univariate regression analysis showed that BB genotype in BsmI gene polymorphism was negatively correlatedwiththeriskofprostatecancer,withstatisticalsignificance(P<0.05,OR=0.321,95%CI=0.181~0.569).Genotype analysis of BB+BB was negatively correlatedwiththeriskofprostatecancer,withstatisticalsignificance(P<0.05,OR=0.410,95%CI=0.237~0.709).Will be confounding factors such as age,smoking history,history of drinking,after adjusting for multiple factors regression analysis,the system shows VDR gene polymorphism sites of BsmI BB genotype and negatively correlated with the risk of prostate cancer,and it has statistical significance(P<0.05,AOR=0.304,95%CI=0.170~0.545),with at least one B alleles of BB and BB genotype is negatively related to the risk of prostate cancer,with statistical significance(P<0.05,AOR=0.669,95%CI=0.560~0.810).The results showed that the BsmI locus of VDR gene polymorphism was significantly correlated with the degree of prostate cancer differentiation(?~2=18.913,P=0.001),and FokI locus was not significantly correlated(?2=2.312,P=0.679).The results showed that the BsmI locus of VDR gene polymorphism was significantly correlated with the degree of prostate cancer differentiation(?~2=18.913,P=0.001),and FokI locus was not significantly correlated(?~2=2.312,P=0.679).The results showed that the polymorphisms of FokI and BsmI genes were significantly correlated with patients'PSA levels(FokI:?~2=17.779,P=0.001;BsmI:?~2=12.667,P=0.013).The results showed that the BsmI site of VDR receptor gene polymorphism was correlated with the presence or absence of metastasis in patients with prostate cancer(?~2=8.679,P=0.013),while the FokI site was not significantly correlated(?~2=1.170,P=0.557).ConclusionsFokI and BsmI sites of vitamin D receptor gene polymorphism were significantly correlated with prostate cancer.It is speculated that the BsmI site of vitamin D receptor gene polymorphism plays an important role in the disease progression of prostate cancer.Figure5;Table11;Reference105.