Oxymatrine Suppresses IL-1?-induced Degradation Of The Nucleus Pulposus Cell And Extracellular Matrix Through TLR4/NF-?B Signaling Pathway

Intervertebral disc(IVD)degeneration(DD)is the primary cause of osphyalgia.Previous studies indicated that inflammation could contribute to nucleus pulposus(NP)cell apoptosis and imbalance between anabolism and catabolism of the NP extracellular matrix(ECM).This research explored the influence of Oxymatrine mitigated NP cells' ECM degradation and apoptosis after IL 1?-induced inflammation,and the possible signaling pathway.We examined gene and protein levels of Collagen II,aggrecan,MMPs(MMP2/3/9/13)and IL-6 in NP cells.The data proved that Oxymatrine mitigated ECM degradation and apoptosis of the NP cells.IL-1?prompted the expression of MMPs and IL-6 through TLR4/NF-?B axis.However,Oxymatrine mitigated the expression of MMPs and TNF-? induced by IL-1?.Moreover,TAK-242 was used as a small molecule inhibitors of TLR-4 signaling to detected the effect of Oxymatrine upon the TLR4/NF-?B signaling.TAK-242 thoroughly inhibited TLR4/NF-?B signaling pathway and increased the level of MMPs and IL-6.These data demonstrate Oxymatrine as a effective medicine that decreased the inflammation in NP cells and degradation of NP tissues.Oxymatrine may be a latent medicine to alleviate disc inflammation and mitigate IDD through TLR4/NF-?B signal pathway.