| Part ? : Characterization and cell experiments of the doxorubicin-loaded Ta@Ca Alg radiopaque microspheres Objective: To characterize the particle size,morphology and developability of microspheres,and to study the biocompatibility of microspheres in cell experiments.Materials and methods: The particle size and morphological changes were detected by electron microscopy and fluorescence microscopy before and after Ta@Ca Alg microspheres were loaded with Dox.The X-ray penetrating ability was detected by Computed Tomography(CT).7402 liver cancer cells were subjected to cytotoxicity experiments using red blood cells for hemolysis experiments.Results: The particle size and morphology of Ta@Ca Alg microspheres do not change significantly after Dox.The developability is not different from the common contrast agent,and the biocompatibility is good.The particle sizes of Ta@Ca Alg and Ta&Dox@Ca Alg were 259±5?m and 257±4?m,respectively.There was no significant difference in size and morphology.The CT values of Ta@Ca Alg microspheres,iodixanol and Ta&Dox@Ca Alg were 3070.0 ±0.6HU,3071.0±0.0HU,3070.8±0.5HU respectively.,no statistical difference(P > 0.05).Ta@Ca Alg,Ta&Dox@Ca Alg cytotoxicity and hemolytic activity are within acceptable limits.Conclusion: The particle size and morphology of Ta@Ca Alg microspheres do not change significantly after Dox.The developability is not different from the common contrast agent,and the biocompatibility is good.Part ?: The drug release characteristics of doxorubicin-loaded Ta@Ca Alg radiopaque microspheres Objective: To evaluate the release process of Ta@Ca Alg microspheres in animals;Materials and Methods:(1)Establish 16 VX2 rabbit liver cancer implantation models,and confirm the successful modeling by liver CT examination;(2)50mg Dox per 1ml microsphere,and Taxy with Ta@Ca Alg microspheres,each treatment.The tumor rabbits used 0.05 mlmicrospheres;(3)4 tumor rabbits were taken from the middle ear artery at 5 minutes,3,6,12,24,and 72 hours after surgery,and the peripheral blood was taken from the middle ear artery,and the plasma was taken by centrifugation;after surgery,4 tumor rabbits were sacrificed at 6,12,24 and 72 hours,and liver,spleen,lung and heart were taken and stored at-80 °C.(4)High-performance liquid Chromatography(HPLC)was used to detect the concentration of Dox in plasma and tumor tissues.The fluorescence distribution of each organ was displayed using a fluorescence imager.Results:(1)Plasma test showed that Dox peaked at 5 minutes and could not be measured after 3 hours;(2)Dox of tumor tissue showed a gradual upward trend within 3 days,and Dox was mainly distributed in liver tumor tissue.Conclusion: Ta@Ca Alg microspheres loaded with Dox TACE treatment can achieve local high concentration release of tumor tissue within 3 days,while peripheral plasma Dox is lower.Part ? pharmacodynamic study of doxorubicin-loaded Ta@Ca Alg radiopaque microspheres in the animal OBJECTIVE: To confirm that Ta@Ca Alg microspheres have triple effects of embolization,chemotherapy and autoradiography when used in TACE therapy.Materials and methods:(1)15 VX2 rabbit liver cancer implantation models were established.The liver model was confirmed by liver CT examination.They were randomly divided into 3 groups,5 in each group.(2)The following treatments were given respectively: Group A(Ta@Ca Alg Microsphere embolization group),group B(Ta&Dox@Ca Alg microsphere embolization group),group C(normal saline pseudoembolic group);(3)Liver-enhanced CT examination was performed 1 day before surgery and 7 days after surgery.The preoperative volume V1 and postoperative volume V2 were measured,and the tumor volume growth rate(V2/V1×100%)of each group was calculated.Tumor rabbits were sacrificed 7 days after CT examination and tumor tissues were sacrificed.Paraffin sections were made and HE staining was performed.RESULTS: All the 15 rabbits were successfully treated with interventional therapy.The embolization process was smooth and the microspheres were developed clearly,which couldaccurately display the position of the microspheres.After 7 days of operation,the tumor growth rate of A and B groups was significantly lower than that of normal saline group,and the durg-eluting microsphere group had better tumor inhibition effect than the blank microsphere group;HE staining results showed that large groups of solidification were observed in groups A and B.There is less necrotic area,and B has fewer residual tumor cells than group A.Conclusion: The Ta@Ca Alg microspheres have good developability during the embolization process and are easy to use.The microsphere drug can inhibit tumor growth better than simple embolization... |
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