DERL1 Regulates Proliferation And Apoptosis Of Human Breast Cancer Cells Via PI3K/Akt Pathway

Objective: To investigate the effect of DERL1 on proliferation and apoptosis of human breast cancer cells ZR-75-1 and MCF-7 and its potential mechanism.Methods:1.Expression of DERL1 in human breast cancer tissues and cells: The expression of DERL1 in human breast cancer and normal tissues was collected and analyzed in the TCGA database.The expression of DERL1 in human breast cancer and normal tissues was detected by real-time quantitative PCR(qRT-PCR)and immunohistochemistry(IHC).The difference in expression of DERL1 in human normal mammary epithelial cells HBL-100 and human breast cancer cells ZR-75-1 and MCF-7 was examined by qRT-PCR and immunocytochemistry(ICC).2.The effect of DERL1 on proliferation and apoptosis of human breast cancer cells: Human breast cancer cells ZR-75-1 and MCF-7 were divided into three groups,namely,DERL1 overexpression group,DERL1 interference group and control group.The DERL1 overexpression plasmid or the DERL1 interference plasmid or the blank control plasmid was transfected into the cells.After transfection for 48 h,the expression of DERL1 in each group was detected by ICC,and then the proliferation ability of each group was detected by crystal violet staining experiment and colony assay.The apoptosis of each group was detected by flow cytometry.The expression of proliferation marker Ki67,Cyclin D,Bcl-2 and apoptosis promoting factor Bax were detected by ICC.3.The mechanism of effect of DERL1 on proliferation and apoptosis of human breast cancer cells: ICC was used to detect the expression of Akt and phosphorylated Akt on the PI3K/Akt pathway in each group.Results:1.The expression of DERL1 in human breast cancer tissues and cells were higher than those in normal tissues and human normal mammary epithelial cells,and the overall survival of patients with higher DERL1 levels was lower;DERL1 has a strong correlation with the important signaling molecules PI3 K and Akt on the PI3K/Akt pathway.2.The DERL1 overexpression plasmid increases the expression of DERL1 protein in human breast cancer cells ZR-75-1 and MCF-7,and the DERL1 interference plasmid can reduce the level of DERL1 protein in the cells.Overexpression of DERL1 can promote the proliferation of human breast cancer cells ZR-75-1 and MCF-7,inhibits apoptosis,and up-regulates the expression of Ki67,Cyclin D and Bcl-2 proteins,and down-regulates the expression of Bax protein.Interfering expression of DERL1 in the cells inhibited cell proliferation and promoted apoptosis,and the expression of Ki67,Cyclin D and Bcl-2 proteins in the cells decreased,and the expression of Bax protein increased.3.Overexpression of DERL1 in human breast cancer cells ZR-75-1 and MCF-7 up-regulates the expression of Akt and phosphorylated Akt in cells.Interfering expression of DERL1 decreased the expression of Akt and phosphorylated Akt in the cells.conclusion: The expression of DERL1 in human breast cancer tissues and human breast cancer cells ZR-75-1 and MCF-7 is higher than that in normal tissues and normal mammary epithelial cells HBL-100,and it activates the PI3K/Akt signaling pathway to affecting cell proliferation and apoptosis.