The Effect Of Detection Method And Intervention Factors Of Bacterial Biofilm In Diabetic Foot Wound

Objective: The aim of this study is to explore a method of biofilm detection combined with clinical practice and experimental dia-gnosis,in an effort to directly reflect the existence of biofilm in chr-onic wounds of patients with diabetes who develop a foot.Methods: Selected 60 patients with diabetic foot who were hospitalized from August 2016 to August 2018 and received negative pressure wound and iodophor irrigation with complete data.Bacterial culture was carried out on the secretion specimens extracted from debridement during the treatment.Gram staining and Azin blue staining were used to observe the wound basal tissue in some cases under op-tical microscope and the basal tissues of the same wound were obse-rved under transmission electron microscopy.All data were input into SPSS24.0 statistical software for statistical analysis.Results: The positive rate of bacterial culture is 58.3%.The mo-st common bacteria are Staphylococcus aureus,Enterococcus faecalis and Staphylococcus epidermidis.Bacterial biofilms are observed under optical and transmission electron microscopes.After the interventio-n of negative pressure wound and iodophor irrigation,the healing rate is 68.3% after 30 days of hospitalization.Conclusion: Bacterial aggregation and the presence of suspected bacterial biofilm in the basement tissue of chronic wound on the diabetic foot can be found under the optical microscope.By virtue of transmission electron microscope,it can be detected that there is a bacterial biofilm in the chronic wound of the diabetic foot.