The Research On Role Of EBLN2 In Hepatochellular Carcinoma

Background &Aim: EBLN2 is an important member of human EBLN family which are recently discovered as non-retroviral elements derived from bornavirus in the genomes of animals.Researchers have found that EBLN2 acts as a candidate gene in cervical cancer and has the interactions with cellar proteins.However,the role of EBLN2 in HCC is dimness.We focus on the functions of EBLN2 in liver cancer.Methods: Quantitive RT-PCR and western blot was used to see the expression level of EBLN2 in HCC tissues and cell lines;Growth curve,colony formation and anchorage-dependent colony formation experiments were used to explore the effect of EBLN2 on HCC cells in vitro when EBLN2 overexpression or knockout.To see the effect of EBLN2 in HCC cells,the cell cycle analysis was performed when EBLN2 overexpression in YY-8103 and Hep G2.Results: We found that EBLN2 m RNA expression was downregulation in HCC samples compared to the corresponding adjacent non-tumorous liver samples and the expression of EBLN2 in most of examined human HCC cells was decreased;EBLN2 overexpression plays an important role in inhibiting ell proliferation,anchorage-dependent and independent colony formation in vitro;Endogenous EBLN2 knockout can significantly promote cell growth,colony formation and soft colony formation of HCC cells;EBLN2 influences cell cycle transition.Conclusions: Our study showed that the expression of EBLN2 was notably downregulated in clinical HCC samples and inhibited HCC cell growth by influencing cell cycle progression in vitro.