Study Of NELL-1 Dual Released Electrospinning Scaffold For The Regeneration Of Cartilage

OBJECTIVE:The short plasma half time and non-osteochondral specification restrict growth factor's application in tissue engineering.This study aims to explore novel osteochondral specific growth factor as well as prolong its release time by designing dual release scaffolds.After that,we studied its effect on cartilage regeneration to provide new methods for the repair of cartilage defect.METHODS:First,we fabricated NELL-1 loaded chitosan nanoparticles.Then the nanoparticles were incorporate into nanofibers by coaxial electrospinning technology.After that,the electrospun nanofibers were twisted into yarn by flowing water vortex technology and orientedly collected by the rotating mandrel.Finally,we assayed the characteristics of the scaffolds for NELL-1 release and stem cells chondrogenesis.RESULTS:Dual release system composed of electrospinning nanofibers and chitosan nanoparticles can significantly prolong growth factor's release time for more than one time.Water vortex collecting technology twists the nanofibers into bundles that can enlarge the pore size of the scaffold to a large extent.The orientedly distributed nanofibers can regulate the array and distribution of the cells to mimic the structure of native cartilage.Slowly and continually releasement of NELL-1 can enhance chondrogenesis and extracellular matrix secretion more efficiently.CONCLUSIONS:.Slowly and continually releasement of growth factor can regulate the differentiation of seed cells better.NELL-1 growth factor can promote the chondrogenic differentiation of stem cells due to its osteochondral specification.