Modifying ?-TCP Composite Scaffolds With Polypeptide To Promote Bone Formation In A Critical-size Rat Calvarial Defect Model

To fabricate the conventional scaffolds with 3D printing technology is an important field in bone tissue engineering study.The objective of this study is to explore the ways of modifying ?-TCP composite scaffolds with polypeptide.Then,the mechanical properties and biological characteristics of the scaffolds are studied.Finally,the model of SD rat skull defects was repaired by the scaffolds,and the osteogenesis ability in vivo was further studied.In this study,POC and ?-TCP were composited to fabricate scaffolds with the 3D printing technology.The POC becomes solidified after crosslinking in the low temperature,so the polypeptide can be alloyed into the scaffolds directly.The activity and distribution of polypeptides are observed.The micro-pore architectures of those scaffolds were observed using optical microscope and scanning electron microscope,and universal material testing machine was used to conduct compressive test on the scaffolds.The water contact angle,cell adhesion and cell proliferation are tested.The osteogenesis ability of the scaffolds is compared in vivo more deeply.The polypeptide remaining active,is evenly distributed in the scaffolds.The relative cell adhesion rate of the scaffolds improves,while the capacity of promoting cell proliferation,the mechanical properties and hydrophilia of the porous scaffolds have not been affected largely,and the capacity of promoting bone formation improves.These research results provide a new idea for the construction of scaffolds for repairing clinical bone defects,and provide a laboratory basis for further clinical application of tissue engineering technology.