Expression Of FBXO32 In Oral Squamous Cell Carcinoma And The Effect On The Biological Behavior Of Oral Squamous Cell Carcinoma

Part one Expression and significance of FBXO32 inoral squamous cell arcinomaObjective:To investigate the expression of FBXO32 in oral squamous cell carcinoma,analyze its relationship with clinicopathological parameters and explore the role of FBXO32 in the progression of oral squamous cell carcinoma.Methods:The expression of FBXO32 gene in oral squamous cell carcinoma and corresponding normal mucosa was detected by qRT-PCR.The paired t-test,chi-square test and continuous calibration chi-square test were used for statistical analysis.The results were P<0.05.The difference was statistically significant.Results:1.In 43 cases of oral squamous cell carcinoma tissues,the relative expression of FBXO32 mRNA is(0.62±0.38),and the relative expression in normal mucosa adjacent to cancer is(1.67±0.85).There is a statistical difference between the two.(P <0.01).2.The expression of FBXO32 mRNA was not correlated with the age and sex of patients with oral squamous cell carcinoma(P>0.05),and was associated with TNM stage,lymph node metastasisand and differentiation degree(P<0.05).The expression of FBXO32 mRNA in stage III+IV was lower than that in stage I+II(P<0.05).The expression of FBXO32 mRNA in the poorly differentiated group was lower than the high-divided group(P<0.05).the expression of FBXO32 mRNA in the lymph node metastasis group was lower than that in the non-lymph node metastasis group(P<0.05).The difference was statistically significant.Conclusions:FBXO32 acts as a tumor suppressor gene in oral squamous cell carcinoma,its expression is associated with the tumor progression and can be used as an important indicator for evaluating the prognosis in patients with oral squamous cell carcinoma.Part two Overexpression of FBXO32 affects the biological behavior of oral squamous cell carcinoma cellsObjective:To detect the expression of FBXO32 in oral squamous cell carcinoma cell lines Tca83 and Cal27,and study the effect of expressioning FBXO32 on the proliferation,migration and invasion of oral squamous cell carcinoma Tca83.Methods:1.qRT-PCR was used to detect the expression of FBXO32 mRNA in Tca83 transfected with oral squamous cell carcinoma cell lines Tca83,Cal27 and myc-FBXO32.2.Western blot analysis of protein expression of Tca83 cells transfected with exogenous FBXO323.The effect of CCK8 proliferation assay on the proliferation of FBXO32 of Tca83 cells was detected.4.The effect of the scratch healing experiment on the migration ability of overexpressing FBXO32 of Tca83 cells.5.Transwell assay detects the effect of overexpression of FBXO32 on the invasive ability of Tca83 cells.Results:1.The relative expression of FBXO32 mRNA in Tca83 and Cal27 cells was(1.01±0.20)and(10.64±0.47),respectively.The relative expression of FBXO32 mRNA in Tca83 cells was lower than that in Cal27.The expression of FBXO32 mRNA in myc-FBXO32 plasmid transfected Tca83 cells was significantly higher than that in the blank group and the control group(P<0.05;P<0.05).2.FBXO32 foreign protein was stably expressed after transfecting the myc-FBXO32 plasmid into Tca83 cells3.The proliferation rate of oral squamous cell carcinoma cell line Tca83 after overexpression of FBXO32 was lower than that of the blank group and the control group,and the difference was statistically significant(P<0.05,P<0.05).4.The horizontal migration velocity of the oral squamous cell carcinoma cell line Tca83 after overexpressing FBX032 was significantly lower(2.34±0.55)than the blank group(5.09±0.52)and the control group(4.99±0.45),the difference was statistically significant(P<0.05,P<0.05).5.The number of longitudinal migration cells of Tca83 cells(53.7±13.5)after overexpression of FBXO32 was significantly lower than that of the blank group(173.0±15.0)and the control group(179.7±6.7),the difference was statistically significant(P<0.05,P < 0.05).After overexpressing FBXO32,the number of cells in the artificial basement membrane(Matrigel)was significantly lower than that in the blank group(111.0±5.6)and the number of control cells(116.7±7.6).The difference was statistically significant(P<0.05,P<0.05).Summery:After overexpression of FBXO32 gene,the proliferation,migration and invasion of Tca83 cells were significantly decreased,indicating that FBXO32 may be involved in the malignant behavior of oral squamous cell carcinoma,such as proliferation,migration and invasion,and can be used as oral cancer.A potential target for gene therapy.