Impact Of Anti-biofilm Drug On Pseudomonas Aeruginosa Based Microbial Fuel Cells

Bacterial biofilm is one of the important mechanisms of the formation of bacterial resistance.Microbial fuel cell(MFC)can convert the chemical energy in the substrate to electrical energy by electricigens.Microbial fuel cell has been widely used in bioremediation,biosensor and wastewater treatment fields.The formation of biofilms in microbial fuel cell plays an important role in the electron transfer between electrogenic bacteria.In this study,we selected Pseudomonas aeruginosa ATCC 9027 as electricigen to establish a microbial fuel cell based on Pseudomonas aeruginosa.We used real-time monitoring of MFC output voltage,bacterial count and activity assay,phenazine concentration determination,anode surface protein content determination and biofilm morphology observation to examine the impact of anti-biofilm drug on Pseudomonas aeruginosa based microbial fuel cell.These result were compared with conventional anti-biofilm drug detection methods,such as crystal violet staining,Scanning electron microscopy(SEM)and Confocal laser scanning microscope(CLSM).The main contents and conclusions are as follows:1.The results of crystal violet staining showed that the biofilm formation of Pseudomonas aeruginosa was inhibited by gold nanoparticle(AuNPs)at the concentration of 10-40 μg/mL.The inhibition was most obvious at the concentration of 10 μg/mL,the amount of biofilm decreased by 22.2% as compared with the control.The biofilm formation of Pseudomonas aeruginosa was increased at the concentration of 80 μg/mL,the amount of biofilm increased by 11.1% as compared with the control.SEM and CLSM results showde that AuNPs could enlarge the bacterial gap in the biofilm.The result of Pseudomonas aeruginosa based MFC showed that the maximum voltage was increased by AuNPs at the concentration of 10-40 μg/mL.The promotion was most obvious at the concentration of 10 μg/mL,the maximum voltage increased by 437.0% as compared with the control.The maximum voltage was inhibited at the concentration of 80 μg/mL,decreased by 25.5% as compared with the control.2.The results of crystal violet staining showed that the biofilm formation of Pseudomonas aeruginosa was increased by silver nanoparticle(AgNPs)at the concentration of 5 μg/mL,the amount of biofilm increased by 16.7% as compared with the control.The biofilm formation of Pseudomonas aeruginosa was inhibited at the concentration of 10-40 μg/mL.The inhibition was most obvious at the concentration of 40 μg/mL,the amount of biofilm decreased by 72.2% as compared with the control.SEM and CLSM results showed that AuNPs could enlarge the bacterial gap in the biofilm.The result of Pseudomonas aeruginosa based MFC showed that the maximum voltage was inhibited by AgNPs at the concentration of 5 μg/mL,the maximum voltage decreased by 31.4%.The maximum voltage was increased at the concentration of 10-40 μg/mL.The promotion was most obvious at the concentration of 10 μg/mL,the maximum voltage increased by 411.6% as compared with the control.As the concentration of AgNPs increase,the startup time of MFC was prolonged.The startup time of MFC was 9.5 h,21 h,53 h and 108 h,respectively.3.The results of crystal violet staining showed that the biofilm formation of Pseudomonas aeruginosa was increased by azithromycin at the concentration of 5 μg/mL,the amount of biofilm increased by 7.4% as compared with the control.The biofilm formation of Pseudomonas aeruginosa was inhibited at the concentration of 80 μg/mL,the amount of biofilm inhibited by 68.6% as compared with the control.SEM and CLSM results show that azithromycin can enlarge the bacterial gap in the biofilm.The result of Pseudomonas aeruginosa based MFC showed that the maximum voltage was increased by azithromycin at the concentration of 10-80 μg/mL.The promotion was most obvious at the concentration of 10 μg/mL,the maximum voltage increased by 437.0% as compared with the control.The maximum voltage was inhibited at the concentration of 80 μg/mL,the maximum voltage decreased by 66.0% as compared with the control.