| Objective:To explore the possible mechanism of EA in treating peripheral nerve injury,we observed the behavioral changes and the expression of SOCS1 in the injured sciatic nerve cells of rats after EA stimulation.Method:(1)75 healthy SD male rats(210—240g)were randomly divided into three groups:control group(n=25): as blank control group,no treatment was given;model group(n=25): after successful modeling,no treatment was given;EA group(n=25): 24 hours after successful modeling,EA treatment was given for 15 minutes,then once a day,5 days a week;acupoint selection: “Zusanli” "YanglingQuan " point(refer to the animal degree atlas),with the needle tip obliquely inward needling about 3-4 mm.(2)Modeling: After intraperitoneal injection of chloral hydrate anesthesia,after successful anesthesia.The animal was supine fixed on the operating table,the operative area was prepared for skin preparation,centered on the middle part of the left femur.The operation area was routinely disinfected by iodophor.Along the middle part of the femur,the incision was about 2-3 cm long.Skin was cut in turn,superficial fascia and piriform muscle were pulled out to expose the scia.Three surgical knots were made from the lower edge of the piriformis muscle about 1 cm,with each surgical knot interval of 0.2 cm and tightness guided.The forefoot was slightly convulsed and sutured layer by layer after completion.(3)The rats in the EA group,model group and control group were sacrificed at 1,2,3,4 and 6 weeks,respectively.The sciatic nerve and spinal cord lumbar 2 to sacral 1segments with a length of about 1 cm in the lower edge of the injured piriformis muscle were taken from the injured side.(4)Record behavioral changes(sciatic nerve index)and weigh the injured triceps surae.(5)Spinal paraffin embedding was done with Nissl staining to observe the number of Nissl bodies.The protein was extracted from sciatic nerve and the expression of SOCS1 protein in sciatic nerve cells was detected by Western blot.Result:(1)Behavioral gait analysis showed that after 1 and 2 weeks,the gait analysis ofmodel group,EA group and control group showed significant difference(P < 0.05)compared with model group and EA group,and there was no significant difference between model group and EA group.After 3 weeks,4 weeks and 6 weeks,the gait analysis of the EA group and the control group showed that the gait of the control group was smaller than that of the model group and the EA group(P < 0.05),and that of the model group was smaller than that of the EA group(P < 0.05).(2)Compared with the EA group and the model group,the value of the control group tended to be 1,with a significant difference(P < 0.05);compared with the EA group and the model group,the EA group tended to be 1,with a significant difference between the two groups(P < 0.05).(3)After Nissl staining,the sciatic neurons in each group were vacuumed under a microscope.Nissl bodies were dark purple and nuclei were light purple(figure 2).The number of sciatic nerve neurons in the model group was significantly lower than that in the control group,and the number of neurons in the EA group was significantly higher than that in the model group.After 6 weeks,the number of neurons in EA group was similar to that in control group.(4)The expression of SOCS1 protein in the control group was lower than that in the EA group and the model group(P < 0.05).The results of 1 and 2 weeks showed that there was no significant difference between the EA group and the model group.(3)The results of 3,4 and 6 weeks showed that the expression of SOCS-1 in EA group was lower than that in model group,with significant difference.Conclusion:Electro-acupuncture stimulation can promote the recovery of nerve function in rats with sciatic nerve injury,which may be related to the expression of SOCS1 in sciatic nerve tissue. |
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