Protective Effect Of Geniposide On A?_1-42-induced SH-SY5Y Cells And Its Relative Mechanism

Objective:To investigate the protective effect of geniposide on A?_1-42-induced SH-SY5Y cells and to explore the mechanism.Methods:In this study,SH-SY5Y cells were induced by 20?mol/L A?_1-42-42 to establish an AD model,and intervention was carried out by pretreatment with geniposide.SH-SY5Y cell lines were divided into four groups:control group,geniposide group,A?_1-42 model group and geniposide protection group.Cell viability was measured by MTT assay.Western blot was used to detect the expression of autophagy-related proteins Beclin1,LC3-I,LC3-II,Atg7 and PI3K/AKT/mTOR pathway proteins p-AKT,AKT,p-mTOR and mTOR.Results:Compared with the control group,the cell viability of SH-SY5Y cells was significantly decreased after incubation with 20?mol/L A?_1-42 for 24h.Compared with the model group,the viability of SH-SY5Y cells in the geniposide protection group was significantly improved.The results of Western blotting showed that compared with the control group,the expression of autophagy-related proteins Atg7,Beclin1 and the ratio of LC3-II/LC3-I in the model group were much reduced.Furthermore,the ratio of PI3K/AKT/mTOR pathway protein p-AKT/AKT and p-mTOR/mTOR were improved in the model group compared to the control group.Compared with the model group,the expression of autophagy-related proteins Atg7,Beclin1 and the ratio of LC3-II/LC3-I in the geniposide protection group was improved.Furthermore,the ratio of PI3K/AKT/mTOR pathway protein p-AKT/AKT and p-mTOR/mTOR in the geniposide protection group compared to the model group.Conclusion:Geniposide has a protective effect on AD cell model,and its mechanism may be related to its inhibition of PI3K/Akt/mTOR pathway and enhancement of autophagy.