Preliminary Study On Antibiotic Resistance Genes Of Gallbladder Calculi,Gallbladder Calculi Combined With Common Bile Duct Calculi And Common Bile Duct Calculi

Objective:To study the distribution of antibiotic resistance genes in patients with cholelithiasis and provide theoretical basis for rational use of antibiotics in clinic.Methods:30 case of gallbladder stones,30 cases of gallbladder stones combined with common bile duct stones and 30 cases of common bile ducts stones were collected.The collected specimens were extracted from bacterial genomes,and the extracted genomes were detected by PCR.Results:Among all detected ARGs,5.92% of the patients with gallbladder stones had the lowest proportion of antibiotics resistance genes to beta-lactam,there were 8 genes of gallstone stone,and the detection rate of strB gene was the highest,27.1%.The detection rates of blaTEM?cfxA?aacc?aadD?aadA1?tetX and sull were 13.9%?5.1%?5.1%?7.7%?20.7%?4.6% and 15.6% respectively.12.94% of the patients with gallbladder stones with common bile duct stones had the lowest proportion of antibiotics resistance genes to beta-lactam,There were 15 genes of gallstone complicated with gallbladder stones with common bile duct stones,the detection rate of sull gene was the highest,17.1%.The detection rates of blaCTX-M ? blaTEM ? blaSHV ? ampC-01 ?ampC/blaDHA?bla-ACC-1?blaGES?cfxA?aac?aacC?aadA1?strB?tetX were 3.2%?9.4%?4.4%?3.4%?8.9%?4.2%?2.8%?2.5%?8.2%?3.9%?8.1%?6.2%?8.2%?8.2%?9.4% respectively.The lowest proportion of aminoglycoside antibiotic resistance genes in patients with common bile duct stones was 17.3%,There were 16 types of choledocholithiasis genes,with the highest detection rate of blaTEM gene,9.1%.The detection rates of blaCTX-M?blaSHV?ampC-01?ampC/blaDHA?bla-ACC-1?blaCMY?blaGES?cfxA?aac?aacC?aadA1?strB?tetX?sull were 7.3%?6.5%?7.6%?5.4%?5.6%?4.0%?4.0%?4.5%?4.7%?6.9%?6.9%?6.1%?6.0%?7.8%?8.9%,respectively.blaCMY was only detected in choledocholithiasis,and aadD was only detected in gallbladder stone.Conclusion:ARGs exist not only in the public environment,but also in the human biliary tract system.At the same time,in the biliary tract system,patients with common bile duct stones carry the most ARGs,followed by patients with common bile duct stones combined with gallbladder stones,and patients with gallbladder stones carry the least ARGs.At the same time,it was found that the lowest level of beta-lactam antibiotic resistance genes was found in patients with gallbladder stones and common bile duct stones combined with gallbladder stones,and the lowest level of aminoglycoside antibiotic resistance genes was found in patients with common bile duct stones.These findings can provide a theoretical basis for the selection of antibiotics in patients with cholelithiasis.