NOP16 Affects Prostate Cancer Progression By Ribosome Synthesis

Objective To detect the expression level of NOP16 in prostate cancer,down-regulate the effect of NOP16 level in prostate cancer cells on the morphology,total protein concentration and biological behavior of prostate cancer cells;down-regulate the function of NOP16 after binding protein complex with NOP16 in vivo The effect of further reduction of NOP16 levels in prostate cancer cells on ribosomal biosynthesis and preliminary discussion of the mechanism.Methods Firstly,the expression levels of NOP16 protein and mRNA in prostate hyperplasia tissue and prostate cancer tissue were detected.Then,specific siRNA was used to knock down the endogenous level of NOP16 cells,and Western Blot and RT-qPCR were used to verify whether knockdown was successful.Using cell count and CCK-8 assay to reduce the level of NOP16 in prostate cancer PC3 cells will affect cell proliferation,using cell scratch test to reduce the expression of NOP16 in prostate cancer cells will affect the migration of cells in vitro,using Transwell Experimental assays have reduced the expression of NOP16 in prostate cancer cells and have an effect on cell invasion in vitro.Using database search,mass spectrometry and co-immunoprecipitation to find proteins that bind to NOP16 and use RT-qPCR and Western-Blot methods to validate NOP16 and binding protein genes in prostate cancer PC3 cell line by specific siRNA.Low,the in vitro behavioral differences of each group were compared.Finally,RT-qPCR was used to detect the difference of 18 S and 28 SrRNA in each group.Results NOP16 in prostate cancer tissues was significantly higher than that in benign prostatic hyperplasia tissues.Transfection of PC3 cells with NOP16 siRNA reduced the expression of endogenous NOP16,and the volume of prostate cancer cells became smaller,the total protein concentration decreased,and the proliferation of prostate cancer cells in vitro.Invasion and migration were inhibited;combined database search,mass spectrometry and co-immunoprecipitation experiments screened the protein hnRNPM which can bind to NOP16 in cells.After reducing the expression of hnRNPM protein,it can promote the invasion and migration of prostate cancer PC3 cells in vitro.But does not affect its proliferation.After knockdown of NOP16 in prostate cancer PC3 cell line with specific siRNA,intracellular 18 S and 28 SrRNA were significantly reduced.Conclusion Low expression of NOP16 in prostate cancer cells can change the size and total protein concentration of prostate cancer cells.At the same time,the proliferation,migration and invasion of prostate cancer cells in vitro will change,and the cell nucleosome size subunits will follow The change of NOP16 changed;the abnormal expression and the protein hnRNPM combined with NOP16 in prostate cancer cells,the migration and invasion of prostate cancer cells changed,but the proliferation changes were not obvious.Therefore,we believe that the expression of NOP16 can affect the development of prostate cancer,and can bind to hnRNPM.Knockdown of NOP16 can affect the role of hnRNPM in prostate cancer,and it affects the occurrence and progress of prostate cancer through ribosome stress.