Anti-lipopolysaccharide Effect Of The Ultrafine Granular Powder Of Houttuynia Cordata Thunb

Objective: To study the anti-lipopolysaccharide(LPS)effect of the ultrafine granular powder of Houttuynia cordata Thunb and the effects in comparison with traditional decoction pieces.Methods: 1.The limulus test method was used to detect the anti-lipopolysaccharide effect of the ultrafine granular powder of Houttuynia cordata Thunb in vivo.2.The mouse model of shock death induced by LPS was used to observe the protective effects of different doses of the ultrafine granular powder of Houttuynia cordata Thunb and traditional decoction pieces on shock death in mice.3.BALB/c mice were injected with LPS through nose to establish lung inflammation model.The number of leukocytes in mice whole blood was examined,and the degree of the inflammation of lung tissue by pathology was observed.4.Rat inflammatory model was induced by injection of LPS into tail vein.The number of leukocytes in mice whole blood,content of LPS in plasma were examined,the content of IL-1?,IL-6 and TNF-? in serum were detected by enzyme-linked immunoadsordent assay(ELISA).5.The rabbits were injected with LPS through marginal ear vein to establish rabbit fever model.The body temperature before and after 4h was measured,the effect of the drugs on the body temperature was observed;after 4h,the number of leukocytes in rabbits whole blood,the content of LPS in plasma were examined,the content of IL-1?,IL-6 and TNF-? in serum were detected by enzyme-linked immunoadsordent assay(ELISA).Result: 1.The ultrafine granular powder of Houttuynia cordata Thunb showed better anti-inflammatory activity than traditional decoction Pieces in vitro at the same concentration and same dilution ratio.2.Compared with the model group,the ultrafine granular powder of Houttuynia cordata Thunb and traditional decoction pieces have a certain protective effect on the death of mice,and the difference is obvious.3.The ultrafine granular powder of Houttuynia cordata Thunb and traditional decoction Pieces can reduce the level of leukocytes in mice whole blood in various degrees,and alleviated the infiltration of inflammatory cells in pathological lung tissue,and there was a positive dosage dependent relation.4.For the two decoction Pieces,the number of leukocytes in rat whole blood,the content of LPS in plasma,the levels of IL-1?,IL-6 and TNF-? in serum decreased in different degrees were found.Compared with traditional decoction Pieces group,the content of IL-1? and TNF-? of serum in ultrafine granular powder group were significant decreased.5.The body temperature and the content of blood leukocyte?lipopolysaccharide(LPS)?IL-1?,IL-6,and TNF-? in model group were significant higher than those in control group.Compared with the model group,The ultrafine granular powder of Houttuynia cordata Thunb could significantly reduce body temperature after 1h in high and medium dose groups;The ultrafine granular powder also decreased the content of blood leukocyte?LPS.Compared with the traditional decoction Pieces group,the ultrafine granular powder significantly reduced body temperature after 1h?the content of blood leukocyte and serum IL-6.The ultrafine granular powder reduced the content IL-1? and TNF-? in some extent,but no significantly difference with the traditional decoction Pieces.Conclusion: The ultrafine granular powder of Houttuynia cordata Thunb has satisfactory anti-lipopolysaccharide effect,and the effect is better than traditional decoction Pieces in some extent,especially in reducing the content of serum inflammatory factors in rats and rabbits and improving the body temperature of rabbits.