The Expression And Clinical Significance Of ARID1A, E-cadherin And EphA2 In Gastric Cancer And Precancerous Lesions

Objective To detect the expression levels of ARID1A,E-cadherin and EphA2 in chronic non-atrophic gastritis,chronic atrophic gastritis with intestinal metaplasia,low-grade intraepithelial neoplasia,high-grade intraepithelial neoplasia,and gastric cancer,and to explore the expression and interaction of ARID1A,E-cadherin and EphA2 in gastric cancer and precancerous lesions,in order to explore effective objective indicators of early diagnosis of gastric cancer,provide a basis for the clinical identification and treatment of early gastric cancer.Methods 1 specimen collection:30 cases of chronic non-atrophic gastritis,30 cases of chronic atrophic gastritis with intestinal metaplasia,30 cases of low-grade intraepithelial neoplasia,30cases of high-grade intraepithelial neoplasia and 30 cases of gastric cancer were selected from the Third Affiliated Hospital of Inner Mongolia Medical University from June 2017 to December 2018.2 Experimental methods:Immunohistochemical staining SP method was used to detect the expression levels of ARID1A,E-cadherin and EphA2 in different gastric mucosa tissues.Results 1 Using the Kruskal Wallis H tests,the expression group rank average of ARID1A in the group of chronic non-atrophic gastritis,chronic atrophic gastritis with intestinal metaplasia,low-grade intraepithelial neoplasia,high-grade intraepithelial neoplasia and gastric cancer were respectively 105.65,95.87,79.33,53.03,43.62,there was a significant difference(?~2=49.188,P<0.01),presenting the gradually decreasing trend.The expression group rank average of E-cadherin in each group were respectively 104.38,96.43,76.68,55.93,44.07,with a significant difference(?~2=46.670,P<0.01),presenting the gradually decreasing trend.The expression group rank average of EphA2 in each group were respectively 34.35,62.32,78.05,99.37,103.42,with a significant difference(?~2=57.488,P<0.01),presenting the gradually inccreasing trend.2 Using the Kendall's tau-b tests to detect the correlation between ARID1A,E-cadherin and EphA2 in each group of the chronic non-atrophic gastritis,chronic atrophic gastritis with intestinal metaplasia,low-grade intraepithelial neoplasia,high-grade intraepithelial neoplasia and and gastric cancer.In each group,ARID1A,E-cadherin were positively correlated,E-cadherin,EphA2 were negatively correlated,ARID1A,EphA2 were negatively correlated,the differences were statistically significant(P<0.05).Conclusion 1 The expression of ARID1A and E-cadherin decreased significantly in the process of gastric mucosal carcinogenesis,suggesting that the absence of ARID1A and E-cadherin expression is closely related to the occurrence and development of gastric cancer,may participate in the early process of gastric cancer,and to some extent reflects the degree of malignant transformation of gastric mucosa.If the expression of ARID1A and E-cadherin is interfered in the early stage of cancer,the incidence of gastric cancer may be reduced.2 EphA2 is significantly increased in the process of gastric mucosal carcinogenesis,suggesting that EphA2 may be involved in the formation of gastric cancer as an oncogene and may be a new molecular marker for the diagnosis of precancerous lesions and early gastric cancer.3 The present study demonstrated that that ARID1A,E-cadherin and EphA2 may interact in the process of gastric mucosal carcinogenesis.In each group,ARID1A is positively correlated with E-cadherin expression,E-cadherin is negatively correlated with EphA2 expression,ARID1A is negatively correlated with EphA2 expression.Among them,ARID1A plays a decisive role,and can directly or indirectly regulate the expression of E-cadherin and EphA2,which is expected to become the stomach new targets for oncogene therapy.