Effects Of L-Ornithine-L-Aspartate On Survival Rates And Liver Function After Different Areas Hepatectomy In Rats

Objective:To establish the 70%,85%,90% rat liver resection model and observe the effect of L-Ornithine-L-Aspartate on survival rates and liver function after liver resection in70%,85%,90% rats.Methods:SD(Sprague-Dawley)rats were selected as experimental animals,and chloral hydrate was intraperitoneally injected with anesthesia at a concentration of 7%.Appropriate improvements were made in the preparation of the hepatectomy model of Higgins rats.The experiment was divided into two parts Part I:Establish the models of 70%,85% and 90% liver resection,and preliminarily observe the survival rates and postoperative survival state.Part II:To observe the changes of mortality and liver function after hepatectomy in different proportions under the intervention of L-Ornithine-L-Aspartateon.Results:Part I:Survival Rates:The survival rates of the 70% hepatectomy model rats at 24 h,48h and 72 h were100%,90%and 90%,respectively.At 24 h,48h and 72 h,the survival rates of 85%hepatectomy model rats was 80%,30%and 0,respectively.The survival rates of 90%hepatectomy model rats at 24 h,48h and 72 h was 0,0 and 0,respectively.Living condition:There was no significant difference between the 70% hepatectomy model group and the sham operation group in terms of postoperative awake time,mental activity,food,water,defecation and other aspects.85% of the rats in the hepatectomy modelgroup had prolonged postoperative awake time,listlessness,lethargy,weak response to external stimuli,dark yellow urine color,and began to die within 24 h,and almost all rats died within 72 h.90% of the rats in the hepatectomy model group were in a coma after the operation,and the recovery time was long.The response to external stimuli basically disappeared,and they died within 24 h..Part II:Survival Rates:None of the rats died at 24 h,48h,and 72 h after 70% hepatectomy combined with L-Ornithine-L-Aspartateon.Accordingly,at 24 h,48h and 72 h,90%,40% and 0 rats survived after 85% hepatectomy combined with L-Ornithine-L-Aspartateon.There were 1,0,and 0 rats survived 24 h,48h,and 72 h after 90% hepatectomy combined with L-Ornithine-L-Aspartateon.Liver function:Serum AST,ALT,BA and TB values of rats in each group increased with the increase of the proportion and time of liver resection,and all biochemical values began to decline after reaching the maximum at 24h(except C1 and C2 groups),showing a single-peak trend.Although the overall trend was similar,the increase of the intervention group was less than that of the control group(P<0.05),which was statistically different.In addition,the biochemical values of the sham operation group were P<0.05 compared with those of the L-Ornithine-L-Aspartateon intervention group and the normal saline control group,showing a statistically significant difference.With the passage of time,the degree of liver damage in rats increased correspondingly.After 12 hours,the arrangement of hepatic cords in rats was slightly disordered,and the cytoplasm was slightly stained.Inflammatory cells began to infiltrate around the portal area,but the structure of hepatic lobules was relatively clear.After 24 hours,the hepatocytes gradually showed watery degeneration,cytoplasmic pale stain,liver cable fracture,mild hepatic sinus pressure,and a large number of inflammatory cells infiltrated the portal area,but no obvious hepatocyte degeneration and necrosis was observed under the microscope.After 48 h,hepatic damage became more serious,and the arrangement of hepatic cells was loose.Microscopically,patchy necrosis,inflammatory exudation,and vacuolar degeneration were more frequently seen,with unclear hepatic lobule structure and obvious expansion of blood sinuses and portal areas.The liver injury of the intervention group was less than that of the normal saline group.Conclusion:1.With the increase of the proportion of liver resection,postoperative mortality of rats increases,and the incidence and severity of postoperative liver failure increases.2.Compared with survival rate and postoperative state,the 85% hepatectomy model in rats is closer to the actual postoperative liver failure state than the 70%hepatectomy model,while the change is more moderate compared with the 90%hepatectomy model,which is convenient for observation and intervention after drug administration.3.L-Ornithine-L-Aspartateon was associated with a 70%,85%,and 90%reduction in mortality after hepatectomy in rats.4.L-Ornithine-L-Aspartateon can reduce the AST,ALT,BA and TB values of the rats by 70%,85% and 90% after hepatectomy,and reduce the incidence of liver failure after hepatectomy.