Correlation Between Expression Of Hif-1 And Functional Status Of CD206 And Its Effect On Tumor Recurrence After Liver Transplantation

OBJECTIVE: To study the expression level of hypoxia-inducible factor-1?(HIF-1?)and tumor-associated macrophage(TAMs)labeled antigen CD206 in liver tissues of patients with tumor recurrence after liver transplantation and the correlation between the two.Provide a basis for tumor recurrence after transplantation.METHODS: A total of 54 patients who underwent liver transplantation at the Organ Transplantation Center of the Affiliated Hospital of Qingdao University from May 2016 to May 2017 were enrolled.The clinical data were complete and pathologically confirmed as hepatocellular carcinoma.There were 47 males and 7 females,aged 35-76 years,with a median age of 53 years.All patients had positive hepatitis B surface antigen and did not receive any chemotherapy,radiotherapy or immunotherapy before surgery.According to the patient's gender,age,tumor size,single/multiple,with or without cancerous thrombus,the patients were classified,and another 54 normal cancer tissues were used as the control group.The expression of HIF-1? and CD206 in 54 cases of hepatocellular carcinoma and adjacent tissues were detected by immunohistochemistry.RESULTS: The positive expression rate of HIF-1? in liver cancer tissues was 48.14%,and the positive expression rate of CD206 in liver cancer tissues was 81.48%,which was significantly higher than that in adjacent tissues(P<0.01).HIF-1?,CD206 and liver cancer tissues The diameter,degree of differentiation,presence or absence of vascular tumor thrombus,tumors were associated with multiple tumors(P<0.05);HIF-1? expression was positively correlated with CD206 expression in liver cancer tissues(r=0.288,P<0.01).HIF-1?,CD206 The probability of tumor recurrence in patients with double positive expression in liver cancer tissues was statistically significant compared with patients with HIF-1? negative and CD206 positive expression.(Log-Rank test ?2=7.309,P=0.007),the probability of tumor recurrence in patients with double positive expression of HIF-1? and CD206 in HCC tissues was statistically different from that of patients with HIF-1? positive and CD206 negative expression.significance.(Log-Rank test ?2=4.545,P=0.033),the probability of tumor recurrence in patients with double positive expression of HIF-1? and CD206 in liver cancer tissues was statistically different from that in patients with double negative expression of HIF-1? and CD206.significance.(Log-Rank test ?2=9.897,P=0.002),while HIF-1? negative,CD206 positive expression,HIF-1? positive,CD206 negative expression,HIF-1?,CD206 double negative expression in liver cancer tissues The recurrence rates were 47.8% vs 60%(?2=0.112,P=0.737),47.8% vs 60%(?2=0.894,P=0.344),60% vs60%(?2=1.317,P=0.251),no difference.Statistical significance.CONCLUSION :HIF-1? and CD206 are highly expressed in hepatocarcinoma tissues,and are related to the degree of differentiation,tumor diameter,multiple presence,and vascular thrombosis.The expression of HIF-1? is positively correlated with the expression of CD206.The probability of tumor recurrence after transplantation in patients with positive expression of HIF-1? and CD206 in liver cancer tissues was significantly higher than that in patients with negative expression.It can be concluded that HIF-1? and CD206 are involved in the occurrence and development of liver cancer.HIF-1? may promote the angiogenesis of liver cancer and promote the metastasis of liver cancer by up-regulating the protein expression of CD206.They have the process of angiogenesis,growth,invasion and metastasis of liver cancer.Superimposed or synergistic.Treatment of liver cancer against HIF-1? and CD206 targets may be a future direction.The combined detection of the two can be used as an auxiliary indicator for the diagnosis of tumor recurrence after liver transplantation.